疟原虫全基因组染色质免疫沉淀测序
Genome-wide chromatin immunoprecipitation-sequencing in Plasmodium.
作者信息
Lopez-Rubio Jose-Juan, Siegel T Nicolai, Scherf Artur
机构信息
Unité de Biologie des Interactions Hôte-Parasite, Institut Pasteur, Paris, France.
出版信息
Methods Mol Biol. 2013;923:321-33. doi: 10.1007/978-1-62703-026-7_23.
Abstract
Chromatin immunoprecipitation (ChIP) studies have been used extensively in recent years to study the functional role of histone marks, variant histones, and other chromatin factors in gene expression in the human malaria parasite, Plasmodium falciparum. In this chapter, we present a ChIP-sequencing protocol optimized for blood-stage forms of this parasite. The processing of the immunoprecipitated DNA prior to high-throughput sequencing is performed in a way to minimize amplification biases due to the high genomic AT-content of the parasite.
摘要
近年来，染色质免疫沉淀（ChIP）研究已被广泛用于研究组蛋白标记、变体组蛋白和其他染色质因子在人类疟原虫恶性疟原虫基因表达中的功能作用。在本章中，我们介绍了一种针对该寄生虫血液阶段形式优化的ChIP测序方案。在进行高通量测序之前，对免疫沉淀的DNA进行处理，以尽量减少由于寄生虫基因组高AT含量导致的扩增偏差。
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