Sinnis Photini, De La Vega Patricia, Coppi Alida, Krzych Urszula, Mota Maria M
Department of Molecular Microbiology and Immunology, John Hopkins Bloomberg School of Public Health, Baltimore, MD, USA.
Methods Mol Biol. 2013;923:385-400. doi: 10.1007/978-1-62703-026-7_27.
There is an important role for in vitro assays to better understand the initial steps of malaria infection. In this section, we describe both microscopy-based and flow cytometry-based sporozoite invasion, migration and development assays with the rodent malaria parasites, Plasmodium berghei and Plasmodium yoelii, and the human malaria parasite, Plasmodium falciparum.
体外试验在更好地理解疟疾感染的初始步骤方面发挥着重要作用。在本节中，我们描述了基于显微镜和流式细胞术的子孢子入侵、迁移和发育试验，这些试验使用的是啮齿类疟原虫伯氏疟原虫和约氏疟原虫，以及人类疟原虫恶性疟原虫。
