通过显微镜检查和流式细胞术对子孢子侵袭、迁移和发育进行定量分析。
Quantification of sporozoite invasion, migration, and development by microscopy and flow cytometry.
作者信息
Sinnis Photini, De La Vega Patricia, Coppi Alida, Krzych Urszula, Mota Maria M
机构信息
Department of Molecular Microbiology and Immunology, John Hopkins Bloomberg School of Public Health, Baltimore, MD, USA.
出版信息
Methods Mol Biol. 2013;923:385-400. doi: 10.1007/978-1-62703-026-7_27.
Abstract
There is an important role for in vitro assays to better understand the initial steps of malaria infection. In this section, we describe both microscopy-based and flow cytometry-based sporozoite invasion, migration and development assays with the rodent malaria parasites, Plasmodium berghei and Plasmodium yoelii, and the human malaria parasite, Plasmodium falciparum.
摘要
体外试验在更好地理解疟疾感染的初始步骤方面发挥着重要作用。在本节中，我们描述了基于显微镜和流式细胞术的子孢子入侵、迁移和发育试验，这些试验使用的是啮齿类疟原虫伯氏疟原虫和约氏疟原虫，以及人类疟原虫恶性疟原虫。
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本文引用的文献
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The malaria circumsporozoite protein has two functional domains, each with distinct roles as sporozoites journey from mosquito to mammalian host.疟原虫环子孢子蛋白有两个功能域，每个功能域在子孢子从蚊子到哺乳动物宿主的旅程中都有不同的作用。
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Role of Plasmodium berghei cGMP-dependent protein kinase in late liver stage development.疟原虫 cGMP 依赖蛋白激酶在肝脏晚期发育阶段的作用。
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Preerythrocytic, live-attenuated Plasmodium falciparum vaccine candidates by design.设计的恶性疟原虫前体红细胞期减毒活疫苗候选物。
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