Vaughan Ashley M, Kappe Stefan H I
Seattle Biomedical Research Institute, University of Washington, Seattle, WA, USA.
Methods Mol Biol. 2013;923:549-66. doi: 10.1007/978-1-62703-026-7_38.
The attenuation of Plasmodium parasites by either radiation or targeted gene deletion can result in viable sporozoites that invade the liver and subsequently arrest. The death of the growth-arrested liver stage parasite and the ensuing recognition by the immune system of parasite antigens promotes protective immunity in immunized mice and humans. The methods described below will enable researchers to determine the efficacy of radiation-attenuated and genetically attenuated rodent malaria sporozoite immunizations against infectious sporozoite challenge, and study protective immunity in immunized mice. In addition, by determining the time of arrest of genetically attenuated parasite liver stages and the mechanisms of clearance, researchers will be able to correlate biological features of the growth-arrested parasites with their ability to promote protective immunity.
通过辐射或靶向基因缺失使疟原虫寄生虫减毒，可产生能够侵入肝脏并随后停滞的活子孢子。生长停滞的肝期寄生虫死亡以及免疫系统随后对寄生虫抗原的识别，可促进免疫小鼠和人类产生保护性免疫。以下所述方法将使研究人员能够确定辐射减毒和基因减毒的啮齿动物疟原虫子孢子免疫对感染性子孢子攻击的效力，并研究免疫小鼠中的保护性免疫。此外，通过确定基因减毒寄生虫肝期的停滞时间和清除机制，研究人员将能够将生长停滞寄生虫的生物学特征与其促进保护性免疫的能力联系起来。
