CNRS EAC 4396, Centre de Recherches Chirurgicales, Faculté de médecine, Université Paris-Est Créteil Val de Marne, Créteil cedex, France.
Tissue Eng Part C Methods. 2013 Apr;19(4):288-98. doi: 10.1089/ten.TEC.2012.0144. Epub 2012 Oct 25.
A novel approach that preserved most mesenchymal stem cell (MSC) characteristics was developed using MSC encapsulation in a hydrogel based on hyaluronic acid (HA). An optimized HA-hydrogel composition, whose characteristics were assessed by scanning electron microscopy and viscoelastic property analyses, as well as the more favorable MSC seeding density, was established. These optimal three-dimensional MSC culture conditions allowed morphological cell remodeling, maintained the expression of stem cell markers over 28 days of culture, and preserved MSC differentiation plasticity. In addition, MSCs in HA-hydrogel submitted for 7 days to mechanical constraint that aimed at mimicking in vivo cardiac beat displayed enhanced cell survival by more than 40% compared to static culture conditions. Thus, the optimized HA-based hydrogel provides a niche for MSCs, which preserves their properties and opens ways for cell therapy, in particular in aortic repair medicine.
开发了一种新方法，通过将间充质干细胞（MSC）包封在基于透明质酸（HA）的水凝胶中来保留大多数 MSC 特征。通过扫描电子显微镜和粘弹性分析评估了优化的 HA-水凝胶组成，并确定了更有利的 MSC 接种密度。这些最佳的三维 MSC 培养条件允许细胞形态重塑，在 28 天的培养过程中保持干细胞标志物的表达，并保持 MSC 分化的可塑性。此外，在 HA-水凝胶中培养 7 天的 MSC 受到旨在模拟体内心跳的机械约束，与静态培养条件相比，细胞存活率提高了 40%以上。因此，优化的基于 HA 的水凝胶为 MSC 提供了一个小生境，保留了它们的特性，并为细胞治疗开辟了道路，特别是在主动脉修复医学中。
