Integrative Medicine Cluster, Advanced Medical and Dental Institute (AMDI), Universiti Sains Malaysia (USM) , Malaysia.
Toxicol Mech Methods. 2013 Feb;23(2):134-43. doi: 10.3109/15376516.2012.730561.
Caffeic acid (CA) (3,4-dihydroxycinnamic acid) is among the major hydroxycinnamic acids. Hydroxycinnamic acid is the major subgroup of phenolic compounds. Methamphetamine (METH) is a potent addictive psychostimulant. Chronic use and acute METH intoxication can cause substantial medical consequences, including spleen, kidney, liver and heart. The objective of the present study was to evaluate the antioxidant activity of CA to protect against oxidative stress and DNA damage to various organs in METH toxicity. Thirty-two male Sprague Dawley (SD) rats were divided into four equal groups: group 1 was injected (i.p) with saline (1 mL/kg) while groups 2,3 and 4 were injected (i.p) with METH (10 mg/kg) twice a day over five days period. Where 100 & 200 mg/kg of CA were injected (i.p) into groups 3 and 4, respectively one day before exposure to METH injections. Tissue antioxidants and DNA content were evaluated in different tissues. METH decreased glutathione (GSH) and glutathione peroxidase (GPx) levels while increased malondialdehyde (MDA), catalase (CAT) and protein carbonyl levels in brain (hypothalamus), liver, and kidney tissues of rats. METH increased hyperdiploidy in these tissues and DNA damage results. Prior treatment of CA to animals exposed to METH restores the above parameters to the normal levels and preserves the DNA content of these tissues. These results were supported by histopathological investigations. In conclusion, METH induced oxidative stress and DNA damage and pretreatment of CA before METH injections prevented tissue oxidative stress and DNA damage in METH-treated animals.
咖啡酸（CA）（3,4-二羟基肉桂酸）是主要的羟基肉桂酸之一。羟基肉桂酸是酚类化合物的主要亚组。甲基苯丙胺（METH）是一种有效的成瘾性精神兴奋剂。慢性使用和急性 METH 中毒会导致严重的医学后果，包括脾、肾、肝和心脏。本研究的目的是评估 CA 的抗氧化活性，以防止 METH 毒性对各种器官的氧化应激和 DNA 损伤。32 只雄性 Sprague Dawley（SD）大鼠被分为四组：第 1 组（i.p）注射生理盐水（1 mL/kg），第 2、3 和 4 组（i.p）每天两次注射 METH（10 mg/kg），共五天。第 3 和 4 组分别在暴露于 METH 注射前一天（i.p）注射 100 和 200 mg/kg 的 CA。评估不同组织中的组织抗氧化剂和 DNA 含量。METH 降低了脑（下丘脑）、肝和肾组织中的谷胱甘肽（GSH）和谷胱甘肽过氧化物酶（GPx）水平，同时增加了丙二醛（MDA）、过氧化氢酶（CAT）和蛋白质羰基水平。METH 增加了这些组织中的超二倍体，并导致 DNA 损伤。预先用 CA 处理暴露于 METH 的动物可将上述参数恢复到正常水平，并保存这些组织的 DNA 含量。这些结果得到了组织病理学调查的支持。总之，METH 诱导氧化应激和 DNA 损伤，而在 METH 注射前用 CA 预处理可防止 METH 处理动物的组织氧化应激和 DNA 损伤。
