Department of Gynecology and Obstetrics, The Third Affiliated Hospital, Sun Yat‑sen University, Guangzhou, Guangdong 510630, PR China.
Oncol Rep. 2012 Dec;28(6):2035-42. doi: 10.3892/or.2012.2038. Epub 2012 Sep 17.
Proline-, glutamic acid- and leucine-rich protein-1/modulator of non-genomic activity of estrogen receptor (ER) (PELP1/MNAR) is a novel nuclear receptor (NR) co-activator that plays an essential role in the actions of ER. Emerging findings suggest that PELP1/MNAR is a novel proto-oncogene, whose expression is deregulated in several hormone-responsive cancers, including endometrial cancer. In this study, we demonstrate that PELP1/MNAR is widely expressed in endometrial carcinoma cell lines. To investigate its possible role in endometrial carcinoma progression, we adopted an RNA interference technology to downregulate PELP1/MNAR expression in Ishikawa endometrial carcinoma cells. PELP1/MNAR downregulation substantially reduced cell proliferation, and the cells in which PELP1/MNAR expression was knocked down also exhibited a decreased migration and invasion ability, as shown by Boyden chamber and invasion assays. The results showed that the expression of MMP-2 and MMP-9 was also decreased. These results suggest that PELP1/MNAR plays a role in endometrial cancer progression and metastasis, and that PELP1/MNAR may be a potential therapeutic target for endometrial cancer.
脯氨酸、谷氨酸和亮氨酸丰富蛋白 1/雌激素受体非基因组活性调节剂（PELP1/MNAR）是一种新型核受体（NR）共激活子，在 ER 作用中发挥着重要作用。新出现的发现表明，PELP1/MNAR 是一种新型原癌基因，其表达在几种激素反应性癌症中失调，包括子宫内膜癌。在这项研究中，我们证明 PELP1/MNAR 在子宫内膜癌细胞系中广泛表达。为了研究其在子宫内膜癌进展中的可能作用，我们采用 RNA 干扰技术下调 Ishikawa 子宫内膜癌细胞中的 PELP1/MNAR 表达。PELP1/MNAR 下调显著降低了细胞增殖，而且敲低 PELP1/MNAR 表达的细胞也表现出迁移和侵袭能力下降，如 Boyden 室和侵袭试验所示。结果表明 MMP-2 和 MMP-9 的表达也降低了。这些结果表明，PELP1/MNAR 在子宫内膜癌的进展和转移中发挥作用，PELP1/MNAR 可能是子宫内膜癌的潜在治疗靶点。
