Emery Benjamin R
Andrology and IVF Laboratories, Division of Urology, Department of Surgery, University of Utah School of Medicine, Salt Lake City, UT, USA.
Methods Mol Biol. 2013;927:167-73. doi: 10.1007/978-1-62703-038-0_15.
Sperm aneuploidy screening has been used as a tool in diagnosis and determining treatment options for male factor infertility since the development of human sperm karyotyping by injection into hamster and mouse oocytes in the 1970s. From these studies and subsequent work with interphase chromosome analysis, at risk populations of men with teratozoospermia, oligozoospermia, and men with translocations, have since been identified. The current technique is an application of fluorescent in situ hybridization (FISH) on interphase sperm nuclei with careful enumeration of the labeled chromosomes to determine sperm ploidy. Typically, five to seven chromosomes are evaluated in individual ejaculates to determine the percent of aneuploid sperm present. This protocol will detail the procedures for: preparation of specimens, exposure of the sperm nuclei to the FISH probes, hybridization, destaining, and scoring criteria.
自20世纪70年代通过将人类精子注射到仓鼠和小鼠卵母细胞中实现人类精子核型分析以来，精子非整倍体筛查一直被用作男性因素不育症诊断和确定治疗方案的工具。从这些研究以及随后的间期染色体分析工作中，此后已确定了患有畸形精子症、少精子症的男性以及患有易位的男性等高危人群。当前技术是荧光原位杂交（FISH）在间期精子核上的应用，通过仔细计数标记染色体来确定精子倍性。通常，在个体射精样本中评估五到七条染色体，以确定非整倍体精子的百分比。本方案将详细介绍以下程序：标本制备、精子核与FISH探针的接触、杂交、脱色以及评分标准。
