Simon Luke, Wilcox Aaron, Carrell Douglas T
Andrology and IVF Laboratories, Division of Urology, Department of Surgery, University of Utah School of Medicine, Salt Lake City, UT, USA.
Methods Mol Biol. 2013;927:247-56. doi: 10.1007/978-1-62703-038-0_22.
Approximately 40% of sterility in couples can be attributed to male subfertility and intracytoplasmic sperm injection (ICSI) has become a powerful tool in assisted reproduction to overcome male infertility. Intracytoplasmic morphologically selected sperm injection (IMSI) is an advanced and sophisticated method of ICSI, where prior to sperm injection the morphology of the sperm is evaluated under high magnification. In addition, the IMSI procedure involves a few minor modifications in sperm preparation which are not carried out during the conventional ICSI procedure, such as the use of MSOME criteria, the requirement for a glass-bottomed dish for selection, prolonged sperm manipulation following separation from the seminal fluid, and sperm storage prior to microinjection. These variations are discussed in this chapter.
约40%的夫妻不育可归因于男性生育力低下，而卵胞浆内单精子注射（ICSI）已成为辅助生殖中克服男性不育的有力工具。卵胞浆内形态学选择精子注射（IMSI）是ICSI的一种先进且复杂的方法，即在精子注射前，在高倍显微镜下评估精子的形态。此外，IMSI程序在精子制备方面涉及一些常规ICSI程序中未进行的小改动，例如使用MSOME标准、需要使用玻璃底培养皿进行选择、从精液中分离后延长精子处理时间以及显微注射前的精子储存。本章将讨论这些差异。
