Department of Otolaryngology, Xiangya Hospital, Central South University, Changsha 410008, Hunan, PR China.
Oncol Rep. 2012 Dec;28(6):2077-82. doi: 10.3892/or.2012.2044. Epub 2012 Sep 19.
Photodynamic therapy has been developed as a viable treatment for cancer, while apoptin is an apoptosis-inducing protein. This study was undertaken to evaluate the feasibility and efficacy of apoptin with photodynamic therapy (PDT) in the treatment of nasopharyngeal carcinoma (NPC). RT-PCR and western blotting were used to detect the expression of apoptin in CNE-2 NPC cells. MTT and flow cytometry analysis were used to detect cell proliferation and cell apoptosis, respectively. Transmission electron microscopy was used to observe cell structures. Hematoxylin and eosin staining was used to observe the xenograft morphology. The expression of apoptin was analyzed by RT-PCR and western blotting in CNE-2 cells stably transfected with PVP3 plasmid. Apoptin restrained cell proliferation and enhanced cell apoptosis compared to controls. Furthermore, we demonstrated that apoptin augmented the effect of PDT with stronger cell proliferation restraint and cell apoptosis and induced severe ultrastructural morphology changes compared to controls. Additionally, we found that the combination of apoptin and PDT produced the strongest inhibition of xenograft growth and tumor necrosis in in vivo experiments. Collectively, we show that apoptin in combination with PDT has a better therapeutic effect in NPC than PDT therapy or apoptin gene therapy alone.
光动力疗法已被开发为治疗癌症的可行方法，而凋亡素是一种诱导细胞凋亡的蛋白质。本研究旨在评估凋亡素联合光动力疗法（PDT）治疗鼻咽癌（NPC）的可行性和疗效。通过 RT-PCR 和 Western blot 检测 CNE-2 NPC 细胞中凋亡素的表达。MTT 和流式细胞术分别用于检测细胞增殖和细胞凋亡。透射电子显微镜用于观察细胞结构。苏木精和伊红染色用于观察异种移植形态。通过转染 PVP3 质粒的 CNE-2 细胞的 RT-PCR 和 Western blot 分析凋亡素的表达。与对照组相比，凋亡素抑制细胞增殖并增强细胞凋亡。此外，我们证明凋亡素增强了 PDT 的效果，与对照组相比，细胞增殖抑制和细胞凋亡更强烈，并诱导严重的超微结构形态变化。此外，我们发现凋亡素与 PDT 的联合应用在体内实验中对异种移植物生长和肿瘤坏死的抑制作用最强。综上所述，我们表明凋亡素联合 PDT 在 NPC 中的治疗效果优于单独的 PDT 治疗或凋亡素基因治疗。
