Akbarzadeh Maryam, Nouri Mohammad, Banekohal Maryam Vahidi, Cheraghi Omid, Tajalli Habib, Movassaghpour Aliakbar, Soltani Sina, Cheraghi Hadi, Feizy Navid, Montazersaheb Soheila, Rahbarghazi Reza, Samadi Nasser
Stem Cell Research Center, Tabriz University of Medical Sciences, Imam Reza St., Golgasht St., 5166614756, Tabriz, Iran.
Department of Clinical Biochemistry and Laboratory Medicine, Tabriz University of Medical Sciences, Imam Reza St., Golgasht St., 5166614756, Tabriz, Iran.
Lasers Med Sci. 2016 Nov;31(8):1565-1572. doi: 10.1007/s10103-016-2016-6. Epub 2016 Jun 30.
The main goal of anti-cancer therapeutic approaches is to induce apoptosis in tumor masses but not in the normal tissues. Nevertheless, the combination of photodynamic irradiation with complementary oncostatic agents contributes to better therapeutic performance. Here, we applied two different cell lines; SKOV3 ovarian carcinoma cells and HUVECs umbilical cord cells as in vitro models to pinpoint whether pharmacological concentration of melatonin in combination with photodynamic therapy induces cell cytotoxicity. The cells were separately treated with various concentrations of melatonin (0 to 10 mM) and photodynamic irradiation alone or in combination. Cells were preliminary exposed to increasing concentrations of melatonin for 24 h and subsequently underwent laser irradiation for 60 s with an output power of 80 mW in continuous mode at 675 nm wavelength and a total light dose of 13.22 J/cm. Cell viability, apoptosis/necrosis rates, and reactive oxygen species levels as well as heat shock protein 70 expression were monitored after single and combined treatments. A statistical analysis was performed by applying one-way analysis of variance (ANOVA) and post hoc Tukey's test. Combination treatment of both cell lines caused a marked increase in apoptosis/necrosis rate, reactive oxygen species generation, and heat shock protein 70 expression compared to incubation of the cells with each agent alone (p < 0.05). SKOV3 cancer cells expressed higher level of heat shock protein 70 under experimental procedure as compared to HUVECs (p < 0.05). Our results introduce melatonin as a potent stimulus for enhancing the efficacy of laser on induction of apoptosis in tumor cells.
抗癌治疗方法的主要目标是诱导肿瘤块中的细胞凋亡，而不是正常组织中的细胞凋亡。然而，光动力照射与互补的抑癌剂联合使用有助于提高治疗效果。在此，我们应用了两种不同的细胞系；SKOV3卵巢癌细胞和人脐静脉内皮细胞（HUVECs）作为体外模型，以确定药理学浓度的褪黑素与光动力疗法联合使用是否会诱导细胞毒性。细胞分别用不同浓度的褪黑素（0至10 mM）单独或联合光动力照射进行处理。细胞先暴露于浓度递增的褪黑素中24小时，随后在675 nm波长下以连续模式、80 mW的输出功率进行60秒的激光照射，总光剂量为13.22 J/cm²。在单一和联合处理后，监测细胞活力、凋亡/坏死率、活性氧水平以及热休克蛋白70的表达。通过应用单因素方差分析（ANOVA）和事后Tukey检验进行统计分析。与单独用每种药物处理细胞相比，两种细胞系的联合处理导致凋亡/坏死率、活性氧生成和热休克蛋白70表达显著增加（p < 0.05）。与HUVECs相比，SKOV3癌细胞在实验过程中热休克蛋白70的表达水平更高（p < 0.05）。我们的结果表明，褪黑素是增强激光诱导肿瘤细胞凋亡疗效的有效刺激物。
