使用剩余 Aptima 样本，比较 GeneXpert CT/NG 检测与 Aptima AC2 检测直肠沙眼衣原体和淋病奈瑟菌的性能。
Performance of the GeneXpert CT/NG assay compared to that of the Aptima AC2 assay for detection of rectal Chlamydia trachomatis and Neisseria gonorrhoeae by use of residual Aptima Samples.
机构信息
Centre for Clinical Infection and Diagnostics Research, Guy's & St. Thomas' NHS Foundation Trust and King's College, London, United Kingdom.
出版信息
J Clin Microbiol. 2012 Dec;50(12):3867-9. doi: 10.1128/JCM.01930-12. Epub 2012 Sep 19.
Abstract
There are currently no commercially available molecular assays for the detection of Chlamydia trachomatis and Neisseria gonorrhoeae in rectal swabs with regulatory approval. We compared the Cepheid GeneXpert CT/NG assay with the GenProbe Aptima Combo2 assay, using 409 rectal swabs. Using Aptima as the gold standard, the sensitivity, specificity, and positive and negative predictive values of GeneXpert for the detection of C. trachomatis and N. gonorrhoeae were 86%, 99.2%, 92.5%, and 98.4% and 91.1%, 100%, 100%, and 98.6%, respectively. Despite significant dilution of samples prior to GeneXpert testing, the assay performed well with excellent specificity.
摘要
目前，尚无经监管批准的可用于直肠拭子中检测沙眼衣原体和淋病奈瑟菌的商业化分子检测方法。我们比较了 Cepheid GeneXpert CT/NG 检测法和 GenProbe Aptima Combo2 检测法，使用了 409 份直肠拭子。以 Aptima 为金标准，GeneXpert 检测沙眼衣原体和淋病奈瑟菌的敏感性、特异性、阳性预测值和阴性预测值分别为 86%、99.2%、92.5%和 98.4%和 91.1%、100%、100%和 98.6%。尽管在进行 GeneXpert 检测之前对样本进行了显著稀释，但该检测方法仍具有出色的特异性，性能良好。
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