Shen Cheng-Huang, Hsieh Hsiao-Yen, Wang Yuan-Hung, Chen Syue-Yi, Tung Chun-Liang, Wu Jiann-DER, Lin Chang-Te, Chan Michael W-Y, Hsu Cheng-DA, Chang Deching
Departments of Medical Research, ; Urology, and ; Institute of Molecular Biology, National Chung Cheng University, Chiayi;
Exp Ther Med. 2010 Sep;1(5):893-898. doi: 10.3892/etm.2010.134. Epub 2010 Jul 21.
Although Dickkopf-1 (DKK1) has been demonstrated to be associated with tumorigenesis in various types of human tumors, a correlation between DKK1 and urothelial carcinoma (UC) has not been reported. In the present study, the correlation between DKK1 expression and UC progression was investigated. Seventy-five UC patients were enrolled. The expression of DKK1 in serum and UC tissue was detected by ELISA, real-time PCR and Western blotting. Prognostic significance was assessed by using Kaplan-Meier survival estimates and log-rank tests. The results showed that serum levels of DKK1 were significantly higher in the UC patients with muscle-invasive (p=0.0001) and high-grade tumors (p=0.00001) as compared to the controls. A high-serum DKK1 was also associated with poor disease-free survival in the UC patients (hazard ratio=2.44; 95% CI 1.10-5.40; p=0.028). Furthermore, DKK1 was also overexpressed in 93% (41/44) of the UC tissues. Therefore, the findings indicate that the expression of DKK1 is associated with UC progression.
尽管Dickkopf-1（DKK1）已被证明与多种类型的人类肿瘤发生相关，但尚未有DKK1与尿路上皮癌（UC）之间相关性的报道。在本研究中，我们调查了DKK1表达与UC进展之间的相关性。纳入了75例UC患者。通过ELISA、实时PCR和蛋白质印迹法检测血清和UC组织中DKK1的表达。使用Kaplan-Meier生存估计和对数秩检验评估预后意义。结果显示，与对照组相比，肌肉浸润性（p = 0.0001）和高级别肿瘤（p = 0.00001）的UC患者血清DKK1水平显著更高。高血清DKK1也与UC患者无病生存期差相关（风险比= 2.44；95%CI 1.10 - 5.40；p = 0.028）。此外，93%（41/44）的UC组织中DKK1也过表达。因此，这些发现表明DKK1的表达与UC进展相关。
