Uozaki Misao, Ikeda Keiko, Tsujimoto Kazuko, Nishide Mitsunori, Yamasaki Hisashi, Khamsri Boonruang, Koyama A Hajime
Division of Virology, Department of Cellular and Molecular Medicine, Wakayama Medical University Graduate School of Medicine, Wakayama 641-0011, Japan ;
Exp Ther Med. 2010 Nov;1(6):983-986. doi: 10.3892/etm.2010.139. Epub 2010 Aug 26.
IN THE PRESENT STUDY, DEHYDROASCORBIC ACID INHIBITED THE MULTIPLICATION OF VIRUSES OF THREE DIFFERENT FAMILIES: herpes simplex virus type 1 (HSV-1), influenza virus type A and poliovirus type 1. Although dehydroascorbic acid showed some cytotoxicity at higher concentrations, the observed antiviral activity was not the secondary result of the cytotoxic effect of the reagent, as the inhibition of virus multiplication was observed at reagent concentrations significantly lower than those resulting in cytotoxicity. Characterization of the mode of the antiviral action of dehydroascorbic acid against HSV-1 revealed that the addition of reagent at any time post infection inhibited the formation of progeny infectious virus in the infected cells, and a one-step growth curve showed that the addition of reagent allowed formation for an additional 2 h, but then almost completely suppressed it. These results indicate that the reagent inhibits HSV-1 multiplication after the completion of viral DNA replication, probably at the step of the envelopment of viral nucleocapsids at the Golgi apparatus of infected cells.
在本研究中，脱氢抗坏血酸抑制了三种不同病毒家族的病毒增殖：1型单纯疱疹病毒（HSV-1）、甲型流感病毒和1型脊髓灰质炎病毒。尽管脱氢抗坏血酸在较高浓度时表现出一定的细胞毒性，但观察到的抗病毒活性并非该试剂细胞毒性作用的次要结果，因为在试剂浓度显著低于导致细胞毒性的浓度时就观察到了病毒增殖的抑制。对脱氢抗坏血酸针对HSV-1的抗病毒作用模式的表征显示，在感染后任何时间添加该试剂均会抑制受感染细胞中子代感染性病毒的形成，一步生长曲线表明，添加该试剂可使病毒形成额外持续2小时，但随后几乎完全抑制其形成。这些结果表明，该试剂在病毒DNA复制完成后抑制HSV-1增殖，可能是在受感染细胞高尔基体处病毒核衣壳包膜形成步骤发挥作用。
