Division of Virology, Department of Cellular and Molecular Medicine, Wakayama Medical University Graduate School of Medicine, Wakayama 641-8509, Japan.
Int J Mol Med. 2010 Mar;25(3):459-63. doi: 10.3892/ijmm_00000365.
N-methyl-pyridinium formate, a novel component of coffee extracts, inhibited the multiplication of both DNA and RNA viruses. In the presence of the compound, the progeny viral yields of both herpes simplex virus type 1 (HSV-1) and poliovirus in HEp-2 cells and those of influenza virus type A in MDCK cells decreased with increasing concentrations of the compound, although the degree of viral sensitivity to this compound differed. In addition, none of these viruses were directly inactivated by the compound at the concentrations tested. Characterization of the mode of action of this compound against HSV-1 multiplication revealed that it inhibits the viral growth primarily at the initial step of virus multiplication, i.e., within 2 h after the onset of multiplication, although the virus multiplication was affected by the compound throughout the multiplication cycle. In addition, this compound showed a significant cytotoxic effect, although the observed antiviral effect was unlikely to be attributed to the cytotoxic effect.
N-甲基吡啶甲酸甲酯，一种新型的咖啡提取物成分，可抑制 DNA 和 RNA 病毒的繁殖。在该化合物存在的情况下，单纯疱疹病毒 1 型（HSV-1）和脊髓灰质炎病毒在 HEp-2 细胞中的子代病毒产量以及甲型流感病毒在 MDCK 细胞中的子代病毒产量随化合物浓度的增加而降低，尽管病毒对该化合物的敏感性程度不同。此外，在测试的浓度下，这些病毒均未被该化合物直接灭活。该化合物对 HSV-1 繁殖的作用方式的特征表明，它主要在病毒繁殖的初始步骤中抑制病毒的生长，即在繁殖开始后 2 小时内，尽管病毒繁殖在整个繁殖周期中都受到该化合物的影响。此外，该化合物显示出明显的细胞毒性作用，尽管观察到的抗病毒作用不太可能归因于细胞毒性作用。
