He Feng-Cheng, Li Shou-Xin, Zhao Zhi-Quan, Dong Jin-Ping, Liu Wu-Zhan, Su Rui-Qiang
Lunan Pharmaceutical Group Co., Ltd., Linyi 276006, China.
Yao Xue Xue Bao. 2012 Jul;47(7):930-3.
The aim of the study is to establish a new method of quality evaluation and validate its feasibilities by the simultaneous quantitative assay of four lignanoids in Schisandra chinensis. A new quality evaluation method, quantitative analysis of multi-components by single marker (QAMS), was established and validated with Schisandra chinensis. Four main lignanoids, schisandrin, schisantherin A, deoxyschizandrin and gamma-schizandrin, were selected as analytes and schisandrin as internal reference substance to evaluate the quality. Their contents in 13 different batches of samples, collected from different bathes, were determined by both external standard method and QAMS. The method was evaluated by comparison of the quantitative results between external standard method and QAMS. No significant differences were found in the quantitative results of four lignanoids in 13 batches of S. chinensis determined by external standard method and QAMS. QAMS is feasible for determination of four lignanoids simultaneously when some authentic standard substances were unavailable, and the developed method can be used for quality control of S. chinensis.
本研究旨在建立一种新的质量评价方法，并通过同时定量测定五味子中的四种木脂素类成分来验证其可行性。建立了一种新的质量评价方法——一测多评法（QAMS），并以五味子进行了验证。选取五味子醇甲、华中五味子酯甲、脱氧五味子素和γ-五味子素这四种主要木脂素类成分作为分析物，以五味子醇甲作为内参物来评价质量。采用外标法和QAMS法分别测定了采自不同产地的13个不同批次样品中它们的含量。通过比较外标法和QAMS法的定量结果对该方法进行评价。外标法和QAMS法测定13批次五味子中四种木脂素类成分的定量结果无显著差异。当缺少某些对照品时，QAMS法可用于同时测定四种木脂素类成分，所建立的方法可用于五味子的质量控制。
