Dou Zhi-hua, An Li-ping, Luo Lin, Ju Yu-feng, Qiao Jin, Chen Min, Miao Ying-xiang
Zhong Yao Cai. 2014 Sep;37(9):1604-8.
To establish a fingerprint of seeds of Schisandra chinensis (SSC) and develop a method of quantitative analysis of multi-components by single marker (QAMS) for simultaneous determining six lignanoids in SSC.
Eleven batches of SSC were determined by HPLC and a common mode of fingerprint has been established. A method was developed for QAMS to determine schizandrol A, schizandrol B, schisantherin A, deoxyschizandrin, schizandrin B and schizandrin C in SSC. Schizandrol A was selected as internal reference; the relative correction factors (RCF)of other five lignanoids to the internal reference were calculated. The contents of the six lignanoids in eleven batches of SSC were determined by both external standard method and QAMS. The QAMS method was evaluated by comparison of its assay results with that of external standard method.
There were 24 common peaks in fingerprints of eleven batches of SSC, six of them were identified. The similarities of fingerprints of eleven batches of SSC were over 0.980. The established RCF had a good reproducibility. No significant differences were found between the quantitative results of external standard method and QAMS.
The developed method is accurate,feasible, and can be used for the qualitative and quantitative analysis of lignanoids in SSC.
建立五味子种子指纹图谱，并建立一种单指标多成分定量分析方法（QAMS），用于同时测定五味子中六种木脂素类成分。
采用高效液相色谱法对11批五味子种子进行测定，建立了通用的指纹图谱模式。建立了一种QAMS方法，用于测定五味子中的五味子醇甲、五味子醇乙、华中五味子酯甲、脱氧五味子素、五味子酯乙和五味子甲素。选择五味子醇甲作为内参物，计算其他五种木脂素类成分相对于内参物的相对校正因子（RCF）。采用外标法和QAMS法分别测定11批五味子种子中六种木脂素类成分的含量。通过比较QAMS法与外标法的测定结果对QAMS法进行评价。
11批五味子种子指纹图谱共有24个共有峰，其中6个峰已鉴定。11批五味子种子指纹图谱相似度均大于0.980。所建立的相对校正因子具有良好的重复性。外标法和QAMS法的定量结果无显著差异。
所建立的方法准确、可行，可用于五味子中木脂素类成分的定性和定量分析。
