Mulle C, Changeux J P
URA CNRS 0210 "Neurobiologie Moleculaire," Départment des Biotechnologies, Institut Pasteur, Paris, France.
J Neurosci. 1990 Jan;10(1):169-75. doi: 10.1523/JNEUROSCI.10-01-00169.1990.
We present a functional characterization of a neuronal nicotinic receptor in the CNS using patch-clamp techniques and a preparation of acutely isolated neurons from the medial habenular nucleus of 10- to 20-d-old rats. The salient pharmacological and electrophysiological properties of this nicotinic response are (1) its association with a channel that is relatively nonselective for cations and has a unitary conductance of 26.2 (+)+/- 5pS at room temperature; (2) its insensitivity to alpha-bungarotoxin and to neuronal bungarotoxin; (3) its activation by the ganglionic nicotinic agonists nicotine, 1,1-dimethyl-4-phenylpiperazinium and cytisine and its blocking by several nicotinic antagonists, mecamylamine, hexamethonium, d-tubocurarine, and dihydro-beta-erythroidine. The combination of these properties has not been reported for any other known type of nicotinic receptor.
我们使用膜片钳技术以及从10至20日龄大鼠内侧缰核急性分离的神经元制备物，对中枢神经系统中的一种神经元烟碱型受体进行了功能特性研究。这种烟碱型反应的显著药理学和电生理学特性包括：（1）它与一种对阳离子相对非选择性的通道相关，在室温下其单通道电导为26.2（+）+/- 5皮西门子；（2）它对α-银环蛇毒素和神经元型银环蛇毒素不敏感；（3）它被神经节烟碱型激动剂尼古丁、1,1-二甲基-4-苯基哌嗪鎓和金雀花碱激活，并被几种烟碱型拮抗剂美加明、六甲铵、d-筒箭毒碱和二氢β-刺桐啶阻断。这些特性的组合在任何其他已知类型的烟碱型受体中均未被报道。
