Li Yong, Zhao Dongyue, Ding Wanlong, Ying Yixin
Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China.
Zhongguo Zhong Yao Za Zhi. 2012 Jun;37(11):1532-5.
In this study, endophytic bacteria were isolated from roots of P. ginseng by plate culture method, and as a result, 40 endophytic bacterial strains were isolated, Bacillus spp. and Pseudomonas spp. were predominant. By confront culture method, two antagonistic endophytic bacterial strain, ge15 (Stenotrophomonas maltophilia) and ge25 (Bacillus sp. ) against Cylindrocarpon destructans, Sclerotinia schinseng and Alternaria pana were identified. The inhibition zone of ge15 to them were 5.5, 22.0, 14.8 mm, respectively; and which were 12.7,16.5,9.0 mm for ge25. The Results indicate that endophytic bacteria have biocontrol potential on ginseng pathogens, and which can be used as a bio-control factor on ginseng soilborne diseases control.
在本研究中，采用平板培养法从人参根部分离内生细菌，结果分离出40株内生细菌菌株，其中芽孢杆菌属和假单胞菌属占主导。通过对峙培养法，鉴定出两株对人参柱孢、人参核盘菌和链格孢具有拮抗作用的内生细菌菌株，即ge15（嗜麦芽窄食单胞菌）和ge25（芽孢杆菌属）。ge15对它们的抑菌圈分别为5.5、22.0、14.8毫米；ge25的抑菌圈分别为12.7、16.5、9.0毫米。结果表明内生细菌对人参病原菌具有生物防治潜力，可作为防治人参土传病害的生物防治因子。
