[基于ISSR标记的云南牛奶菜属几种药用植物的亲缘关系]
[Genetic relationship on several medicinal plants in Marsdenia from Yunnan in ISSR marker].
作者信息
Guan Zhibin, Zhang Lixia, Song Meifang, Li Haitao, Zhang Zhonglian
机构信息
Yunnan Branch of Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Jinghong 666100, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2012 Jun;37(11):1550-2.
OBJECTIVE
To analyze the genetic relationship of 9 Marsdenia species from Yunnan, especially the traditional Dai medicine "Dai Bai Jie" (M. auricularis).
METHOD
Applying the inter-simple sequence repeats (ISSR) markers technology.
RESULT
Twenty-five primers were screened out of 60 ISSR random primers and produced 391 bands totally, every primer produce 8-21 bands and the mean number was 15.6. The range of the GS (genetic similarity) value was 0.6675-0.8210. In 9 Marsdenia species, M. auricularis is a relative of M. tenacissima. M. balansae and M. officinalis have the closest genetic relationship.
CONCLUSION
It is supported by ISSR that the M. auricularis which is sib species of M. tenacissima, and the folk medicine of Marsdenia are worthy deep investigation and study.
目的
分析云南9种牛奶菜属植物的亲缘关系，尤其是传统傣药“傣白解”（耳叶牛奶菜）。
方法
采用简单重复序列区间（ISSR）标记技术。
结果
从60条ISSR随机引物中筛选出25条，共产生391条带，每条引物产生8 - 21条带，平均为15.6条。遗传相似性（GS）值范围为0.6675 - 0.8210。在9种牛奶菜属植物中，耳叶牛奶菜与通光散有亲缘关系，云南牛奶菜和紫花牛奶菜的亲缘关系最为密切。
结论
ISSR分析支持耳叶牛奶菜与通光散为近缘种，牛奶菜属民间药用植物值得深入研究。
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