Liu Xia, Liu Zheng, Yang Min, Chen Ke-Li
School of Chemical Engineering, Wuhan University of Technology, Wuhan 430079, China.
Zhong Yao Cai. 2010 Jul;33(7):1052-5.
To study the identification of Liriope spicata var. prolifera and its affinis species, which are difficult to be differentiated with routine method, based on ISSR molecular marker technology and explore their relationship.
According to the pre-experiment, to design orthogonal experiment of four levels and five factors to optimize the PCR reaction system. To select randomly 80 primers from ISSR primers sequence table and then screen effective primers from the experiment trough PCR amplifying of all samples. Mark the samples with optimal ISSR conditions, and calculate the similarity coefficient between samples, then build the phylogenetic tree of these samples.
The study established a better ISSR reaction system and the PCR amplification procedure for Liriope spicata var. prolifera and its affinis species, screened out 9 effective primers and achieved ISSR electrophoretic spectrum and phylogenetic tree of 15 samples.
The established ISSR molecular marker techniques can be used for the researches of identification and genetic relationship for some Liriope and Ophiopogon species.
基于ISSR分子标记技术研究难以用常规方法区分的麦冬及其近缘种的鉴别，并探讨它们之间的关系。
根据预实验，设计四水平五因素的正交实验以优化PCR反应体系。从ISSR引物序列表中随机选取80条引物，然后通过对所有样品进行PCR扩增，从实验中筛选出有效引物。用最佳ISSR条件对样品进行标记，计算样品间的相似系数，然后构建这些样品的系统发育树。
本研究建立了较好的麦冬及其近缘种的ISSR反应体系和PCR扩增程序，筛选出9条有效引物，获得了15个样品的ISSR电泳图谱和系统发育树。
所建立的ISSR分子标记技术可用于部分麦冬属和沿阶草属植物的鉴别及亲缘关系研究。
