Li Erna, Teng Zaijin, Liu Shiping, Qin Song, Zhang Cang
Nanjing Sanhome pharmaceutical Co., Ltd., Nanjing 210038, China.
Zhongguo Zhong Yao Za Zhi. 2012 Jun;37(11):1610-3.
To establish a HPLC-ELSD fingerprint of Marsdenia tenacissima from different habitats, in order to provide a reliable method for scientific assessment and effective quality control.
HPLC-ELSD was adopted to determine 25 baches of M. tenacissima herbs from different habitats. Traditional Chinese medicine chromatographic fingerprint similarity software assessment system 2004 developed by China Pharmacopoeia Committee was adopted to establish a common mode chart and assess chromatographic similarity based on the degree of correlation.
The common mode for M. tenacissima herb C21 steroidal fingerprint was established, including 11 common characteristic peaks. Among them, 10 were identified. According to the assessment on the similarity of 25 batches of samples, 80% of them showed a similarity of over 0.80 in steroidal HPLC-ELSD fingerprint.
The method can be used to assess the quality of M. tenacissima.
建立不同产地通关藤的高效液相色谱-蒸发光散射检测器（HPLC-ELSD）指纹图谱，为其科学评价及有效质量控制提供可靠方法。
采用HPLC-ELSD法测定25批不同产地的通关藤药材。采用中国药典委员会开发的《中药色谱指纹图谱相似度评价系统2004》建立共有模式图谱，并基于相关性程度对色谱相似度进行评价。
建立了通关藤药材C21甾体指纹图谱的共有模式，包括11个共有特征峰。其中，10个已被鉴定。根据对25批样品相似度的评价，80%的样品在甾体HPLC-ELSD指纹图谱中相似度超过0.80。
该方法可用于评价通关藤的质量。
