Girod C, Dubois M P, Trouillas J
C R Seances Soc Biol Fil. 1979;173(3):535-41.
Indirect immunofluorescence technique with anti-17-39ACTH and anti beta-endorphin sera has allowed us to detect "corticotropic cells" in the anterior and intermediate lobes of the adenohypophysis of the male cat. The corticotropic cells of the anterior lobe are localized in the median zone; they are PAS-positive and appeared intensively coloured in dark blue with the Herlant's tetrachrome. All the cells of the intermediate lobe react with the anti-17-39ACTH serum. Using an anti-beta-endorphin serum, we have observed that all the corticotropic cells of the anterior lobe react; but in the intermediate lobe, only a part of "corticotropic cells" react with the anti-beta-endorphin serum.
使用抗17 - 39促肾上腺皮质激素(ACTH)和抗β - 内啡肽血清的间接免疫荧光技术,使我们能够在雄性猫腺垂体的前叶和中间叶中检测到“促肾上腺皮质激素细胞”。前叶的促肾上腺皮质激素细胞位于中间区域;它们对过碘酸雪夫(PAS)呈阳性反应,并用赫兰特四色染色法染成深蓝色。中间叶的所有细胞都与抗17 - 39ACTH血清发生反应。使用抗β - 内啡肽血清时,我们观察到前叶的所有促肾上腺皮质激素细胞都发生反应;但在中间叶中,只有一部分“促肾上腺皮质激素细胞”与抗β - 内啡肽血清发生反应。