Mulvogue H M, McMillen I C, Robinson P M, Perry R A
J Dev Physiol. 1986 Oct;8(5):355-68.
An immunocytochemical staining technique was used to localize four fragments [pro gamma MSH, gamma MSH, ACTH and beta endorphin/beta lipotrophin (beta endorphin/beta LPH)] of the proopiomelanocortin molecule in both the adult and fetal sheep pituitary. In the adult sheep anterior pituitary each fragment was localized in cells that were darkly stained, stellate and widely distributed throughout the gland. The same cells, identified in three serial sections, stained with anti-pro gamma MSH, anti-ACTH and anti-beta endorphin/beta LPH. In the fetal sheep anterior pituitary all the proopiomelanocortin derived fragments were present at 38 days gestation. Between about 90 and 130 days of gestation both adult type proopiomelanocortin cells (small, stellate) and uniquely fetal cells (large, columnar) were present. Both adult-type and fetal proopiomelanocortin cells were identified in serial sections of the fetal anterior pituitary, stained with anti-pro gamma MSH, anti-ACTH and anti-beta endorphin/beta LPH. The adult intermediate lobe was immunoreactive with anti-pro gamma MSH and anti-beta endorphin/beta LPH but not with anti-gamma MSH or anti-ACTH. The fetal intermediate lobe was immunoreactive with all four antisera from 60 days gestation.
采用免疫细胞化学染色技术，在成年和胎羊垂体中定位阿黑皮素原分子的四个片段[前γ促黑素、γ促黑素、促肾上腺皮质激素和β内啡肽/β促脂解素（β内啡肽/β促脂解素）]。在成年羊垂体前叶中，每个片段都定位于深色染色、呈星状且广泛分布于整个腺体的细胞中。在连续的三个切片中鉴定出的相同细胞，用抗前γ促黑素、抗促肾上腺皮质激素和抗β内啡肽/β促脂解素进行染色。在妊娠38天时，胎羊垂体前叶中存在所有源自阿黑皮素原的片段。在妊娠约90至130天时，既有成年型阿黑皮素原细胞（小的、星状），也有独特的胎儿细胞（大的、柱状）。在胎羊垂体前叶的连续切片中，用抗前γ促黑素、抗促肾上腺皮质激素和抗β内啡肽/β促脂解素进行染色，鉴定出了成年型和胎儿型阿黑皮素原细胞。成年羊垂体中间叶对抗前γ促黑素和抗β内啡肽/β促脂解素有免疫反应，但对抗γ促黑素或抗促肾上腺皮质激素无免疫反应。从妊娠60天起，胎羊垂体中间叶对所有四种抗血清均有免疫反应。
