CIMAR/CCMAR, Centre of Marine Sciences of Algarve, University of Algarve, Campus de Gambelas, 8005-139, Faro, Portugal.
J Agric Food Chem. 2012 Oct 24;60(42):10613-24. doi: 10.1021/jf3023244. Epub 2012 Oct 16.
The quality and shelf life of fish meat products depend on the skeletal muscle's energetic state at slaughter, as meat decomposition processes can be exacerbated by energy depletion. In this study, we tested dietary glycerol as a way of replenishing muscle glycogen reserves of farmed gilthead seabream. Two diets were tested in duplicate (n = 42/tank). Results show 5% inclusion of crude glycerol in gilthead seabream diets induces increased muscle glycogen, ATP levels and firmness, with no deleterious effects in terms of growth, proximate composition, fatty acid profile, oxidative state, and organoleptic properties (aroma and color). Proteomic analysis showed a low impact of glycerol-supplementation on muscle metabolism, with most changes probably reflecting increased stress coping capacity in glycerol-fed fish. This suggests inclusion of crude glycerol in gilthead seabream diets (particularly in the finishing phase) seems like a viable strategy to increase glycogen deposition in muscle without negatively impacting fish welfare and quality.
鱼肉制品的质量和保质期取决于屠宰时骨骼肌的能量状态，因为能量枯竭会加剧肉的分解过程。在这项研究中，我们测试了甘油作为补充养殖金头鲷肌肉糖原储备的一种方法。两种饲料各进行了两次重复测试（n = 42/缸）。结果表明，在金头鲷饲料中添加 5%的粗甘油可增加肌肉糖原、ATP 水平和硬度，在生长、基本成分、脂肪酸谱、氧化状态和感官特性（香气和颜色）方面没有不良影响。蛋白质组学分析表明，甘油补充对肌肉代谢的影响较小，大多数变化可能反映了甘油喂养的鱼增加了应对压力的能力。这表明在金头鲷饲料中添加粗甘油（特别是在育肥阶段）似乎是一种可行的策略，可以增加肌肉中的糖原沉积，而不会对鱼类福利和质量产生负面影响。
