CIMAR/CCMAR, Centre of Marine Sciences of Algarve, University of Algarve, Faro, Portugal.
J Agric Food Chem. 2012 Sep 19;60(37):9443-53. doi: 10.1021/jf301766e. Epub 2012 Sep 10.
Fish welfare is an important concern in aquaculture, not only due to the ethical implications but also for productivity and quality-related reasons. The purpose of this study was to track soluble proteome expression in post-mortem gilthead seabream muscle and to observe how preslaughter stress affects these post-mortem processes. For the experiment, two groups of gilthead seabream (n = 5) were subjected to distinct levels of preslaughter stress, with three muscle samples being taken from each fish. Proteins were extracted from the muscle samples, fractionated, and separated by 2DE. Protein identification was performed by MALDI-TOF-TOF MS. Analysis of the results indicates changes on several cellular pathways, with some of these changes being attributable to oxidative and proteolytic activity on sarcoplasmic proteins, together with leaking of myofibrillar proteins. These processes appear to have been hastened by preslaughter stress, confirming that it induces clear post-mortem changes in the muscle proteome of gilthead seabream.
鱼类福利是水产养殖中的一个重要关注点，这不仅是因为涉及伦理问题，还因为与生产力和质量有关。本研究的目的是跟踪死后真鲷肌肉中可溶性蛋白质组的表达，并观察宰前应激如何影响这些死后过程。为此，将两组真鲷（n = 5）置于不同程度的宰前应激下，从每只鱼中取出三个肌肉样本。从肌肉样本中提取蛋白质，进行分级，然后通过 2DE 分离。通过 MALDI-TOF-TOF MS 进行蛋白质鉴定。结果分析表明，几个细胞途径发生了变化，其中一些变化归因于肌浆蛋白的氧化和蛋白水解活性，以及肌原纤维蛋白的渗漏。这些过程似乎因宰前应激而加速，证实宰前应激会导致真鲷肌肉蛋白质组发生明显的死后变化。
