Liu Dong-Fu, Wu Ji-Tao, Wang Jian-Ming, Liu Qing-Zuo, Gao Zhen-Li, Liu Yun-Xiang
Department of Urology, Yantai Yuhuangding Hospital, Yantai, China.
Asian Pac J Cancer Prev. 2012;13(7):3313-7. doi: 10.7314/apjcp.2012.13.7.3313.
Prostate cancer is a highly prevalent disease in older men of the western world. MicroRNAs (miRNAs) are small RNA molecules that regulate gene expression via posttranscriptional inhibition of protein synthesis. To identify the diagnostic potential of miRNAs in prostate cancer, we downloaded the miRNA expression profile of prostate cancer from the GEO database and analysed the differentially expressed miRNAs (DE-miRNAs) in prostate cancerous tissue compared to non-cancerous tissue. Then, the targets of these DE-miRNAs were extracted from the database and mapped to the STRING and KEGG databases for network construction and pathway enrichment analysis. We identified a total of 16 miRNAs that showed a significant differential expression in cancer samples. A total of 9 target genes corresponding to 3 DE-miRNAs were obtained. After network and pathway enrichment analysis, we finally demonstrated that miR-20 appears to play an important role in the regulation of prostate cancer onset. MiR-20 as single biomarker or in combination could be useful in the diagnosis of prostate cancer. We anticipate our study could provide the groundwork for further experiments.
前列腺癌在西方老年男性中是一种高度流行的疾病。微小RNA（miRNA）是一类小RNA分子，通过对蛋白质合成的转录后抑制来调控基因表达。为了确定miRNA在前列腺癌中的诊断潜力，我们从基因表达综合数据库（GEO数据库）下载了前列腺癌的miRNA表达谱，并分析了前列腺癌组织与非癌组织中差异表达的miRNA（DE-miRNA）。然后，从数据库中提取这些DE-miRNA的靶标，并映射到STRING和京都基因与基因组百科全书（KEGG）数据库进行网络构建和通路富集分析。我们共鉴定出16种在癌症样本中表现出显著差异表达的miRNA。获得了与3种DE-miRNA相对应的总共9个靶基因。经过网络和通路富集分析，我们最终证明miR-20似乎在前列腺癌发病机制的调控中发挥重要作用。miR-20作为单一生物标志物或联合使用可能对前列腺癌的诊断有用。我们预计我们的研究可为进一步的实验奠定基础。
