Department of Biological Sciences, University of Alberta, Edmonton, AB, Canada.
Mol Ecol Resour. 2012 Nov;12(6):1145-50. doi: 10.1111/1755-0998.12017. Epub 2012 Sep 21.
Recent advances in technology facilitated development of large sets of genetic markers for many taxa, though most often model or domestic organisms. Cross-species application of genomic technologies may allow for rapid marker discovery in wild relatives of taxa with well-developed resources. We investigated returns from cross-species application of three commercially available SNP chips (the OvineSNP50, BovineSNP50 and EquineSNP50 BeadChips) as a function of divergence time between the domestic source species and wild target species. Across all three chips, we observed a consistent linear decrease in call rate (~1.5% per million years), while retention of polymorphisms showed an exponential decay. These results will allow researchers to predict the expected amplification rate and polymorphism of cross-species application for their taxa of interest, as well as provide a resource for estimating divergence times.
近年来，技术的进步促进了大量遗传标记的发展，这些标记适用于许多分类群，尽管大多数是模式生物或家养生物。基因组技术的跨物种应用可以在资源丰富的分类群的野生近缘种中快速发现标记。我们研究了三种商业上可用的 SNP 芯片（绵羊 SNP50、牛 SNP50 和马 SNP50 BeadChips）在与家养来源物种和野生目标物种分化时间之间的跨物种应用的回报。在所有三种芯片中，我们观察到呼叫率（~每百万年 1.5%）呈一致的线性下降，而多态性的保留则呈指数衰减。这些结果将使研究人员能够预测他们感兴趣的分类群的跨物种应用的预期扩增率和多态性，并提供估计分化时间的资源。
