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氮饥饿对九种微藻中三酰基甘油积累动态的影响。

The impact of nitrogen starvation on the dynamics of triacylglycerol accumulation in nine microalgae strains.

机构信息

Bioprocess Engineering, Wageningen University and Research Centre, Wageningen, The Netherlands.

出版信息

Bioresour Technol. 2012 Nov;124:217-26. doi: 10.1016/j.biortech.2012.08.003. Epub 2012 Aug 10.

DOI:10.1016/j.biortech.2012.08.003
PMID:22995162
Abstract

Microalgae-derived lipids are an alternative to vegetable and fossil oils, but lipid content and quality vary among microalgae strains. Selection of a suitable strain for lipid production is therefore of paramount importance. Based on published results for 96 species, nine strains were selected to study their biomass, total fatty acid, and triacylglycerol (TAG) production under nitrogen-sufficient and deficient cultivation conditions. Under nitrogen-deficient conditions, Chlorella vulgaris, Chlorella zofingiensis, Neochloris oleoabundans, and Scenedesmus obliquus, accumulated more than 35% of their dry weight as TAGs. Palmitic and oleic acid were the major fatty acids produced. The main difference between these strains was the amount of biomass that was produced (3.0-7.8-fold increase in dry weight) and the duration that the biomass productivity was retained (2-7 days) after nitrogen depletion. S. obliquus (UTEX 393) and C. zofingiensis (UTEX B32) showed the highest average TAG productivity (322 and 243 mg l(-1)day(-1)).

摘要

微藻衍生的油脂是植物油和矿物油的替代品,但不同微藻菌株的油脂含量和质量差异很大。因此,选择适合生产油脂的菌株至关重要。基于已发表的 96 种物种的结果,选择了 9 种菌株,在氮充足和缺乏的培养条件下研究其生物量、总脂肪酸和三酰基甘油(TAG)的生产。在氮缺乏条件下,普通小球藻、中兴小球藻、南海盐藻和斜生栅藻积累了超过 35%的干重作为 TAG。棕榈酸和油酸是主要的脂肪酸。这些菌株的主要区别在于生物量的产生量(干重增加 3.0-7.8 倍)和氮耗尽后生物量生产力的保持时间(2-7 天)。斜生栅藻(UTEX 393)和中兴小球藻(UTEX B32)表现出最高的平均 TAG 生产力(322 和 243 mg l(-1)day(-1))。

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