College of Horticulture, Jilin Agricultural University, Changchun 130118, PR China.
Gene. 2012 Dec 10;511(1):54-61. doi: 10.1016/j.gene.2012.09.021. Epub 2012 Sep 17.
Blueberry (Vaccinium spp.) is an important small fruit crop rich in antioxidants. However, tissue-specific transcriptome and genomic data in public databases are not sufficient for an understanding of the molecular mechanisms associated with antioxidants, especially the biosynthesis of anthocyanins. Here, we obtained more than 64 million sequencing reads from blueberry skin and pulp using Illumina sequencing technology. De novo assemblies yielded 34,464 unigenes, among them 1236 transcripts and 862 putative transcription factors involved in the main antioxidant biosynthesis pathway were identified. Comparative transcript profiling allowed the identification of 92 differentially expressed genes with potential relevance in regulating the fruit metabolism and anthocyanin content during ripening. A series of qRT-PCR confirmed the high expression level of the anthocyanin pathway genes in the skin of the blue fruit from the in silico study. This sequence collection provides a significant resource for the blueberry research and breeding work.
蓝莓（Vaccinium spp.）是一种富含抗氧化剂的重要小水果。然而，公共数据库中组织特异性转录组和基因组数据不足以了解与抗氧化剂相关的分子机制，特别是花色苷的生物合成。在这里，我们使用 Illumina 测序技术从蓝莓果皮和果肉中获得了超过 6400 万条测序reads。从头组装生成了 34464 条 unigenes，其中鉴定出 1236 个与主要抗氧化剂生物合成途径相关的转录本和 862 个假定转录因子。比较转录谱分析鉴定出 92 个差异表达基因，它们可能与调控果实成熟过程中的代谢和花色苷含量有关。一系列 qRT-PCR 实验从计算机研究中证实了花色苷途径基因在蓝莓果皮中的高表达水平。该序列集为蓝莓研究和育种工作提供了重要资源。
