School of Pharmacy, Faculty of Health Sciences, University of Eastern Finland, Yliopistonranta 1, P.O. Box 1627, Kuopio 70211, Finland.
J Neurosci Methods. 2012 Nov 15;211(2):218-26. doi: 10.1016/j.jneumeth.2012.09.011. Epub 2012 Sep 17.
Carbon fiber electrodes (CFE) are commonly used for in vivo detection of catecholamines due to their excellent electrochemical properties and biocompatibility. Fast-scan cyclic voltammetry (FSCV) combined with CFEs permits the detection of catecholamines such as dopamine (DA) with high specificity and reliability. However, advances in neuroscience constantly demand sensors with greater sensitivities and selectivities. This study investigated an untreated CFE of 32 μm diameter and 300 μm exposed length for detection of DA using FSCV. Despite the larger area of the working electrode, we observed only a small increase in the background current in comparison with the commonly used CFE of 7 μm diameter and 100 μm exposed length. The sensitivity of the 32 μm CFE was 9 times greater than that of the 7 μm CFE. These larger electrodes exhibited good linearity and a 6 fold higher signal-to-noise ratio than 7 μm CFEs in vitro. The 32 μm CFE showed significantly better selectivity for DA in preference to 3,4-dihydroxyphenylacetic acid than the 7 μm CFE and similar selectivity to the 7 μm CFE for ascorbic and homovanillic acid. The electrodes displayed good temporal resolution and electrochemical stability in both in vitro and in vivo tests.
碳纤维电极（CFE）由于其出色的电化学性能和生物相容性，常用于体内儿茶酚胺的检测。快速扫描循环伏安法（FSCV）与 CFE 结合使用，可以高特异性和可靠性地检测儿茶酚胺，如多巴胺（DA）。然而，神经科学的不断进步始终需要具有更高灵敏度和选择性的传感器。本研究使用 FSCV 对 32 μm 直径和 300 μm 暴露长度的未经处理的 CFE 进行了 DA 的检测。尽管工作电极的面积更大，但与常用的 7 μm 直径和 100 μm 暴露长度的 CFE 相比，我们观察到背景电流仅略有增加。32 μm CFE 的灵敏度比 7 μm CFE 高 9 倍。这些较大的电极在体外表现出良好的线性和 6 倍的信号噪声比，优于 7 μm CFE。32 μm CFE 对 DA 的选择性明显优于 7 μm CFE 对 3,4-二羟基苯乙酸的选择性，与 7 μm CFE 对抗坏血酸和高香草酸的选择性相似。电极在体内和体外测试中均显示出良好的时间分辨率和电化学稳定性。
