State Key Laboratory of Pulp and Paper Engineering, South China University of Technology, Guangzhou 510006, Guangdong, China.
J Biotechnol. 2012 Dec 31;162(2-3):183-90. doi: 10.1016/j.jbiotec.2012.09.006. Epub 2012 Sep 17.
A comparative study was made of Mung bean epoxide hydrolases-catalyzed asymmetric hydrolysis of styrene oxide to (R)-1-phenyl-1,2-ethanediol in an n-hexane/buffer biphasic system containing various hydrophilic ionic liquids (ILs). Compared to the n-hexane/buffer biphasic system alone, addition of a small amount of hydrophilic ILs reduced the amount of non-enzymatic hydrolysis, and improved the reaction rate by up to 22%. The ILs with cation containing an alkanol group, namely [C(2)OHMIM][BF(4)] and [C(2)OHMIM][TfO], and the choline amino acid ILs [Ch][Arg] and [Ch][Pro] were found to be the most suitable co-solvents for the reaction, owing to their good biocompatibility with the enzyme, which led to high initial rates (0.99-1.25 μmol/min) and high product e.e.s (95%). When substrate concentration was around 30 mM, where optimal performance was observed with the IL-containing systems, the product e.e. was improved from 90% without ILs to ≥95% in the presence of ILs.
在含有各种亲水离子液体（ILs）的正己烷/缓冲双相体系中，对绿豆环氧水解酶催化的苯乙烯氧化物不对称水解为（R）-1-苯基-1,2-乙二醇进行了比较研究。与单独的正己烷/缓冲双相体系相比，添加少量亲水 IL 可减少非酶水解的量，并将反应速率提高多达 22%。具有含烷醇基团的阳离子的 IL，即 [C(2)OHMIM][BF(4)] 和 [C(2)OHMIM][TfO]，以及胆碱氨基酸 IL [Ch][Arg] 和 [Ch][Pro]，被发现是反应最适合的共溶剂，因为它们与酶具有良好的生物相容性，从而导致高初始速率（0.99-1.25 μmol/min）和高产物对映体过量值（95%）。当底物浓度约为 30 mM 时，在含有 IL 的体系中观察到最佳性能，产物对映体过量值从无 IL 时的 90%提高到有 IL 时的≥95%。
