Department of Cardiac Surgery, Zhongshan Hospital, Fudan University & Shanghai Institute of Cardiovascular Diseases, No. 180 Fenglin Road, Xuhui District, Shanghai, People's Republic of China.
Inflamm Res. 2013 Jan;62(1):69-79. doi: 10.1007/s00011-012-0553-4. Epub 2012 Sep 21.
To investigate whether Th17/Treg imbalance exists, and whether VEGF(165) attenuates the imbalance in allogeneic skeletal myoblast transplantation (allo-SMT) for acute myocardial infarction (AMI).
On days 1, 2, 4, and 7 after allo-SMT, the percentages and ratios of Th17 and Treg cells were analyzed by flow cytometry in three groups-the AMI group, the AMI-S group (allo-SMT) and the AMI-V group (with VEGF(165) treatment). Subsequently, related proinflammatory and regulatory cytokines and key transcription factors, ROR-γt mRNA and Foxp3 mRNA expression, were examined by Bio-plex and real-time polymerase chain reaction, respectively.
On days 1, 2, 4, and 7, the percentage of Tregs, related cytokine concentrations and transcript factor Foxp3 mRNA in the AMI-S group were lower than those in the AMI group, while those in the AMI-V group were higher than those in the AMI group. However, the percentage of Th17 cells, related cytokine concentrations and ROR-γt mRNA in the AMI-S group were higher than those in the AMI group; those in the AMI-V group were lower than those in the AMI group. Compared with the AMI group, the ratios of Th17/Treg cells significantly increased in the AMI-S group and decreased in the AMI-V group.
Th17/Treg imbalance participated in the formation and development of the inflammatory and immune response after allo-SMT. However, transfected VEGF(165) was able to relieve the severity of the Th17/Treg imbalance.
研究 Th17/Treg 失衡是否存在，以及 VEGF(165) 是否会减轻同种异体骨骼肌移植（allo-SMT）治疗急性心肌梗死（AMI）后的失衡。
在 allo-SMT 后第 1、2、4 和 7 天，通过流式细胞术分析三组（AMI 组、AMI-S 组（allo-SMT）和 AMI-V 组（VEGF(165)治疗）中 Th17 和 Treg 细胞的百分比和比值。随后，通过 Bio-plex 和实时聚合酶链反应分别检测相关促炎和调节细胞因子以及关键转录因子 ROR-γt mRNA 和 Foxp3 mRNA 的表达。
在第 1、2、4 和 7 天，AMI-S 组的 Treg 百分比、相关细胞因子浓度和转录因子 Foxp3 mRNA 均低于 AMI 组，而 AMI-V 组则高于 AMI 组。然而，AMI-S 组的 Th17 细胞百分比、相关细胞因子浓度和 ROR-γt mRNA 均高于 AMI 组；AMI-V 组则低于 AMI 组。与 AMI 组相比，AMI-S 组的 Th17/Treg 细胞比值显著增加，而 AMI-V 组则降低。
Th17/Treg 失衡参与了 allo-SMT 后炎症和免疫反应的形成和发展。然而，转染的 VEGF(165)能够缓解 Th17/Treg 失衡的严重程度。
