Department of Pathology, Korea University Guro Hospital, Korea University College of Medicine, Seoul, Korea.
J Surg Oncol. 2013 Apr;107(5):505-10. doi: 10.1002/jso.23271. Epub 2012 Sep 20.
Aberrant expressions of specific microRNAs are recently known in many malignancies, including gastric carcinoma. The prognostic implication of oncogenic microRNA dysregulation was investigated in advanced gastric carcinomas undergoing radical resection and adjuvant systemic chemotherapy, and observed on long-term follow-up.
The expression levels of miR-20a, miR-21, miR-25, miR-93, miR-103, miR-106a, miR-106b, miR-130b, miR-155, miR-221, and miR-222 were analyzed in formalin-fixed paraffin-embedded (FFPE) cancer tissues of 91 patients, using reverse transcription real-time PCR.
The high expression of miR-20a, miR-25, miR-93, miR-103, miR-106a, miR-106b, miR-130 was associated with lymph node metastasis (P < 0.05), and high expression of miR-155 was related to tumor penetration through serosa and lymph node metastasis (P < 0.05). Cases with high expression of miR-222 (P = 0.014) showed reduced 5-year survival rates. The high expression of miR-222 and miR-221 showed correlation with shorter metastasis-free survival (P = 0.039 and 0.033, respectively), and miR-222 high expression was related to reduced overall survival (P = 0.012).
The high expression of miR-20a, miR-25, miR-93, miR-103, miR-106a, miR-106b, miR-130, miR-155, miR-221, and miR-222 in AGC tissues may be a high risk factor associated with tumor penetration through serosa, lymph node metastasis, distant metastasis, and poor long-term survival in patients undergoing radical resection and adjuvant systemic chemotherapy.
特定 microRNA 的异常表达最近在许多恶性肿瘤中被发现，包括胃癌。本研究旨在探讨在接受根治性切除和辅助全身化疗的晚期胃癌患者中，致癌 microRNA 失调的预后意义，并进行长期随访。
采用逆转录实时 PCR 分析 91 例胃癌患者福尔马林固定石蜡包埋（FFPE）组织中 miR-20a、miR-21、miR-25、miR-93、miR-103、miR-106a、miR-106b、miR-130b、miR-155、miR-221 和 miR-222 的表达水平。
miR-20a、miR-25、miR-93、miR-103、miR-106a、miR-106b 高表达与淋巴结转移相关（P<0.05），miR-155 高表达与浆膜穿透和淋巴结转移相关（P<0.05）。miR-222 高表达（P=0.014）的病例 5 年生存率降低。miR-222 和 miR-221 高表达与无转移生存时间较短相关（P=0.039 和 0.033），miR-222 高表达与总生存时间缩短相关（P=0.012）。
在接受根治性切除和辅助全身化疗的 AGC 患者中，miR-20a、miR-25、miR-93、miR-103、miR-106a、miR-106b、miR-130、miR-155、miR-221 和 miR-222 在胃癌组织中的高表达可能是与浆膜穿透、淋巴结转移、远处转移和接受根治性切除和辅助全身化疗的患者长期生存不良相关的高危因素。
