Kleinschmidt A M, Pederson T, Tani T, Ohshima Y
Cell Biology Group, Worcester Foundation for Experimental Biology, Shrewsbury, MA 01545.
J Mol Biol. 1990 Jan 5;211(1):7-9. doi: 10.1016/0022-2836(90)90005-7.
A Schizosaccharomyces pombe U6 small nuclear RNA gene containing an intron has been described. We find that the S. pombe U6 gene is transcribed in a human (HeLa) cell S100 extract with an alpha-amanitin sensitivity characteristic of RNA polymerase III. The S. pombe U6 gene is also transcribed after transfection into human cells. The transcription of vertebrate U6 RNA genes by RNA polymerase III does not require intragenic control elements. The intron of the S. pombe U6 gene disrupts a "box A"-like intragenic sequence that is typically an RNA polymerase III transcription control element. This, together with the transcription of the S. pombe U6 gene by human RNA polymerase III, suggests that it is recognized by human U6 gene-specific transcription machinery.
已描述了一种含有内含子的粟酒裂殖酵母U6小核RNA基因。我们发现粟酒裂殖酵母U6基因在人（HeLa）细胞S100提取物中以RNA聚合酶III的α-鹅膏蕈碱敏感性特征进行转录。粟酒裂殖酵母U6基因在转染到人细胞后也会转录。RNA聚合酶III对脊椎动物U6 RNA基因的转录不需要基因内控制元件。粟酒裂殖酵母U6基因的内含子破坏了一个通常为RNA聚合酶III转录控制元件的“框A”样基因内序列。这一点，再加上人RNA聚合酶III对粟酒裂殖酵母U6基因的转录，表明它被人U6基因特异性转录机制所识别。
