Graham D J, Alexander J J
Department of Surgery, Case Western Reserve University School of Medicine, Cleveland Metropolitan General Hospital, OH 44109.
J Vasc Surg. 1990 Feb;11(2):307-12; discussion 312-3. doi: 10.1067/mva.1990.17098.
Recent evidence suggests that thrombin interacts with various cell types, stimulating cellular proliferation and protein and prostanoid production. To further delineate its role in vascular healing, we have studied the effects of thrombin on proliferation and matrix production by the cells of the vessel wall. The addition of thrombin (1 unit/ml) to cultures of bovine aortic smooth muscle cells resulted in an increase in cell proliferation (p less than 0.01) and number (p less than 0.03), whereas in cultures of bovine aortic endothelial cells thrombin produced a decrease in cell proliferation (p less than 0.01) and number (p less than 0.02). Thrombin also altered matrix composition in cultures of these cells. In both bovine aortic endothelial cells and bovine aortic smooth muscle cell cultures grown in the presence of thrombin, total protein content was significantly increased when compared to controls (p less than 0.03). In bovine aortic endothelial cell cultures the addition of thrombin resulted in a decrease in collagen content (p less than 0.01) and an increase in sulfated glycosaminoglycan content (p less than 0.02). In contrast, in bovine aortic smooth muscle cell cultures thrombin resulted in an increase in collagen content (p less than 0.03), whereas glycosaminoglycan content was unaffected. These findings suggest that thrombin may significantly influence vascular healing and function by altering cell number and matrix composition.
近期证据表明，凝血酶可与多种细胞类型相互作用，刺激细胞增殖以及蛋白质和前列腺素的产生。为了进一步阐明其在血管愈合中的作用，我们研究了凝血酶对血管壁细胞增殖和基质产生的影响。向牛主动脉平滑肌细胞培养物中添加凝血酶（1单位/毫升）会导致细胞增殖增加（p<0.01）和细胞数量增加（p<0.03），而在牛主动脉内皮细胞培养物中，凝血酶会导致细胞增殖减少（p<0.01）和细胞数量减少（p<0.02）。凝血酶还改变了这些细胞培养物中的基质组成。在存在凝血酶的情况下培养的牛主动脉内皮细胞和牛主动脉平滑肌细胞培养物中，与对照组相比，总蛋白含量均显著增加（p<0.03）。在牛主动脉内皮细胞培养物中添加凝血酶会导致胶原蛋白含量减少（p<0.01）和硫酸化糖胺聚糖含量增加（p<0.02）。相反，在牛主动脉平滑肌细胞培养物中，凝血酶会导致胶原蛋白含量增加（p<0.03），而糖胺聚糖含量不受影响。这些发现表明，凝血酶可能通过改变细胞数量和基质组成来显著影响血管愈合和功能。
