Moredo Fabiana A, Cappuccio Javier A, Insarralde Lucas, Perfumo Carlos J, Quiroga María A, Leotta Gerardo A
Catedra de Microbiología, Facultad de Ciencias Veterinarias, UNLP, Calle 60 y 118, (1900) La Plata, Pcia. de Buenos Aires, Argentina.
Rev Argent Microbiol. 2012 Apr-Jun;44(2):85-8.
The purpose of this work was to characterize 47 Escherichia coli strains isolated from 32 pigs diagnosed with postweaning diarrhea and three pigs with edema disease by PCR. Forty two (95.5 %) of the strains isolated from diarrheic pigs were characterized as enterotoxigenic E. coli (ETEC) and 2 (4.5 %) as Shiga toxin-producing E. coli (STEC). Fourteen (33.3 %) ETEC strains were positive for est/estII/fedA genes. The most complex genotype was eltA/estI/faeG/aidA. Strains isolated from pigs with ED were classified as porcine STEC and were stx2e/aidA carriers. Eleven (25 %) strains carried the gene encoding adhesin protein AIDA-I. However, genes coding for F5, F6, F41, intimin and Paa were not detected. The development of vaccines generating antibodies against prevalent E. coli adhesins in Argentina could be useful for the prevention of PWD and ED.
本研究旨在通过PCR对从32头诊断为断奶后腹泻的猪和3头发病仔猪水肿病的猪中分离出的47株大肠杆菌进行特征分析。从腹泻猪中分离出的菌株有42株（95.5%）被鉴定为产肠毒素大肠杆菌（ETEC），2株（4.5%）为产志贺毒素大肠杆菌（STEC）。14株（33.3%）ETEC菌株的est/estII/fedA基因呈阳性。最复杂的基因型是eltA/estI/faeG/aidA。从水肿病猪中分离出的菌株被归类为猪源STEC，并且是stx2e/aidA携带者。11株（25%）菌株携带编码粘附蛋白AIDA-I的基因。然而，未检测到编码F5、F6、F41、intimin和Paa的基因。开发针对阿根廷流行的大肠杆菌粘附素产生抗体的疫苗可能有助于预防断奶后腹泻和水肿病。
