实时 PCR 用于区分仔猪腹泻和水肿病中产肠毒性和志贺毒素大肠杆菌 F18 变异体。
Real-time PCR for differentiation of F18 variants among enterotoxigenic and Shiga toxin-producing Escherichia coli from piglets with diarrhoea and oedema disease.
机构信息
Animal Disease Diagnostic Division, Animal and Plant Quarantine Agency, 175 Anyangro Manangu, Anyang 430-757, Republic of Korea; College of Veterinary Medicine, Chungbuk National University, Cheongju 361-763, Republic of Korea.
出版信息
Vet J. 2013 Nov;198(2):538-40. doi: 10.1016/j.tvjl.2013.07.021. Epub 2013 Aug 28.
One-step real-time PCR using one set of primers and four probes was developed for differentiation of F18 variants (F18 common, F18ab, F18ac, F18new variant) of enterotoxigenic (ETEC) and Shiga toxin-producing (STEC) Escherichia coli from piglets with diarrhoea and oedema disease. The limits of detection for F18common, F18ab, F18ac, and F18new variant were 10(7), 10(7), 10(5) and 10(7)colony forming units/g faeces, respectively. Of 94 Korean isolates of E. coli encoding F18, 70 were F18ac (43 STEC/ETEC, 4 STEC and 23 ETEC), 15 were F18ab (all STEC) and nine were F18new variant (1 STEC/ETEC, 7 STEC, 1 ETEC).
建立了一种一步实时 PCR 方法，使用一套引物和四个探针，用于区分仔猪腹泻和水肿病中产肠毒素（ETEC）和志贺毒素产生（STEC）大肠杆菌的 F18 变体（F18 常见、F18ab、F18ac、F18 新变体）。F18 常见、F18ab、F18ac 和 F18 新变体的检测限分别为 10(7)、10(7)、10(5)和 10(7)cfu/g 粪便。在 94 株韩国大肠杆菌 F18 编码株中，70 株为 F18ac（43 株 STEC/ETEC、4 株 STEC 和 23 株 ETEC），15 株为 F18ab（均为 STEC），9 株为 F18 新变体（1 株 STEC/ETEC、7 株 STEC、1 株 ETEC）。
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