Degese María F, Cabrera Marta G, Krivokapich Silvio J, Irazu Lucia E, Rodríguez Marcelo A, Guarnera Eduardo A
Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán" Instituto Nacional de Enfermedades Infecciosas, Departamento de Parasitología. Av. Vélez Sarsfield 563 (1281) Ciudad Autónoma de Buenos Aires, Argentina.
Rev Argent Microbiol. 2012 Apr-Jun;44(2):97-100.
Mansonella ozzardi is a tissue-dwelling parasitic nematode, the causative agent of mansonelliasis in almost all Latin American countries. It has been described along the Argentine Yungas region. The microscopic diagnosis can yield false-negative test results at low microfilaremia levels. The aim of this study was to optimize the molecular diagnostic technique and compare it with the Knott's method and standard blood smear procedures (thin blood films and thick smears) in 92 blood samples of individuals from an endemic area. The PCR technique followed by the sequencing of the amplified product yielded 100 % sensitivity compared to the Knott's test, which is considered a reference method. Seven more cases of this parasitosis could only be identified with the molecular technique.
奥氏曼森线虫是一种寄生于组织内的寄生线虫，几乎在所有拉丁美洲国家都是曼氏线虫病的病原体。它已在阿根廷的永加斯地区被描述过。在微丝蚴血症水平较低时，显微镜诊断可能会得出假阴性检测结果。本研究的目的是优化分子诊断技术，并将其与来自流行地区的92份个体血样中的Knott氏法和标准血涂片程序（薄血膜和厚涂片）进行比较。与被视为参考方法的Knott氏试验相比，PCR技术及其扩增产物测序的灵敏度为100%。另外7例这种寄生虫病病例只能通过分子技术鉴定出来。
