Gelbícová T, Karpísková R
Masarykova univerzita, Ustav experimentální biologie, Ceská sbírka mikroorganismů, Brno.
Klin Mikrobiol Infekc Lek. 2012 Apr;18(2):38-42.
Evaluation of the incidence and characteristics of L. monocytogenes in samples of raw cow's milk collected on farms (bulk tank milk samples) and from vending machines.
Detection of L. monocytogenes and enumeration were carried out according to EN/ISO 11290--1, 2. Strains were characterised by serotyping and macrorestriction analysis using pulsed-field gel electrophoresis.
The presence of L. monocytogenes was detected in 3,2 % (11/346) of bulk tank milk samples and 1,8 % (4/219) samples of raw cow's milk from vending machines. Findings of L. monocytogenes in raw milk were sporadic. Only on one farm strains of L. monocytogenes were detected repeatedly. Thirteen strains of L. monocytogenes belonged to serotype 1/2a, two strains to serotype 1/2b and one to serotype 4b. Macrorestriction analysis revealed considerable heterogeinity of profiles, with nine different pulsotypes being detected. Pulsotype 711 was the most frequent. This pulsotype was found on three different farms.
The incidence of L. monocytogenes in raw cow's milk is relatively low in the Czech Republic. The results confirmed that some clones of L. monocytogenes from raw milk are identical with food and human strains.
评估农场采集的生牛奶样本（大罐牛奶样本）和自动售货机中单核细胞增生李斯特菌的发生率及特征。
根据EN/ISO 11290 - 1、2进行单核细胞增生李斯特菌的检测和计数。通过血清分型和使用脉冲场凝胶电泳的宏观限制性分析对菌株进行鉴定。
在3.2%（11/346）的大罐牛奶样本和1.8%（4/219）的自动售货机中生牛奶样本中检测到单核细胞增生李斯特菌。生牛奶中单核细胞增生李斯特菌的检出情况是零星的。仅在一个农场多次检测到单核细胞增生李斯特菌菌株。13株单核细胞增生李斯特菌属于血清型1/2a，2株属于血清型1/2b，1株属于血清型4b。宏观限制性分析显示图谱存在相当大的异质性，检测到9种不同的脉冲型。脉冲型711最为常见。该脉冲型在三个不同农场被发现。
在捷克共和国，生牛奶中单核细胞增生李斯特菌的发生率相对较低。结果证实，来自生牛奶的一些单核细胞增生李斯特菌克隆与食品和人类菌株相同。
