Anses, Unité Hygiéne et Qualité des Produits Avicoles et Porcins, BP53, 22440 Ploufragan, France.
J Food Prot. 2012 May;75(5):889-95. doi: 10.4315/0362-028X.JFP-11-340.
This study was undertaken to acquire new data on the prevalence of Listeria monocytogenes in sows and fattening pigs in farrow-to-finish pig farms, and to analyze distribution of serotypes and genotypes of the bacterium within farms. Detection of L. monocytogenes was carried out on 730 pooled feces samples from sows in 73 pig farms and on 172 pooled feces samples from fattening pigs in 43 of these farms. Isolates were serotyped and typed by pulsed-field gel electrophoresis. For sows, 46% of the farms and 11% of the samples were positive for L. monocytogenes. A total of 124 isolates were collected and distributed in four serotypes: 1/2a (41%), 1/2b (36%), 4b (21%), and 1/2c (2%). Positive farms harbored one to three serotypes. The genetic diversity was high; 51 genetic profiles were obtained with 25, 16, 9, and 1 for the serotypes 1/2a, 1/2b, 4b, and 1/2c, respectively. Positive farms harbored 1 to 6 genetic profiles. Isolates showing similar genotypes occurred in several farms. For fattening pigs, 25% of the farms and 14.5% of the samples were positive for L. monocytogenes. The 34 isolates belonged to four serotypes: 1/2a (32%), 1/2b (41%), 4b (24%), and 1/2c (3%). They were distributed in 20 genotypes: 6 for 1/2a; 8 for 1/2b, 5 for 4b, and 1 for 1/2c. Similar serotypes and pulsotypes were recovered in sows and fattening pigs from the same farms, suggesting common sources of contamination.
本研究旨在获取有关分娩至育肥猪场中母猪和育肥猪中李斯特菌单核细胞增生李斯特菌流行率的新数据，并分析该细菌在农场内的血清型和基因型分布。在 73 个猪场的 730 份母猪粪便混合样本和其中 43 个猪场的 172 份育肥猪粪便混合样本中进行了李斯特菌检测。通过脉冲场凝胶电泳对分离株进行血清分型和分型。对于母猪，46%的猪场和 11%的样本呈李斯特菌单核细胞增生李斯特菌阳性。共采集了 124 株分离株，分布在四个血清型：1/2a（41%）、1/2b（36%）、4b（21%）和 1/2c（2%）。阳性农场携带一种到三种血清型。遗传多样性很高；获得了 51 种遗传谱，血清型 1/2a、1/2b、4b 和 1/2c 分别为 25、16、9 和 1。阳性农场携带 1 到 6 种遗传谱。具有相似基因型的分离株出现在几个农场中。对于育肥猪，25%的猪场和 14.5%的样本呈李斯特菌单核细胞增生李斯特菌阳性。34 株分离株属于四个血清型：1/2a（32%）、1/2b（41%）、4b（24%）和 1/2c（3%）。它们分布在 20 种基因型中：1/2a 为 6 种；1/2b 为 8 种，4b 为 5 种，1/2c 为 1 种。来自同一农场的母猪和育肥猪中回收了相似的血清型和脉冲型，表明存在共同的污染来源。
