Jalil Rufaihah Abdul, Neng Lee Chuen, Kofidis Theodoros
Department of Surgery, Yong Loo Lin School of Medicine, National University of Singapore, Singapore.
J Stem Cells. 2011;6(2):93-9.
Human pluripotent stem cells such as embryonic and induced pluripotent stem cells have the ability to differentiate into all cell types representing the adult human body which includes vascular endothelial cells. Vascular endothelial cells are of great interest because of the huge role that they play in tissue regeneration and engineering. They can be used in cell therapy for the treatment of cardiovascular diseases such as myocardial infarction and peripheral arterial diseases and also in tissue engineering in the form of vascular grafts or vascularized tissue constructs before transplantation. In order for these applications to be realized, well-defined, efficient and reproducible differentiation protocols are important in directing stem cells differentiation into endothelial cells followed by stringent purification and expansion of pure populations of these desired cells in vitro. This review focuses on various well-characterized differentiation procedures that have been used to derive endothelial cells from human pluripotent stem cells and the challenges faced in using these cells in clinical applications.
人类多能干细胞，如胚胎干细胞和诱导多能干细胞，具有分化为代表成人体的所有细胞类型的能力，其中包括血管内皮细胞。血管内皮细胞因其在组织再生和工程中发挥的巨大作用而备受关注。它们可用于细胞治疗，以治疗心血管疾病，如心肌梗死和外周动脉疾病，也可用于组织工程，以血管移植物或血管化组织构建体的形式在移植前使用。为了实现这些应用，明确、高效且可重复的分化方案对于引导干细胞分化为内皮细胞，随后在体外严格纯化和扩增这些所需细胞的纯群体至关重要。本综述重点关注已用于从人类多能干细胞中获得内皮细胞的各种特征明确的分化程序，以及在临床应用中使用这些细胞所面临的挑战。
