Luo Zhu-Lin, Tian Fu-Zhou, Tang Li-Jun, Wang Tao, Ren Jian-Dong, Yan Hong-Tao, Huang Zhu, Wan Hua
Department of General Surgery, Chengdu General Military Hospital, Chengdu 610083, China.
Sichuan Da Xue Xue Bao Yi Xue Ban. 2012 Jul;43(4):540-2, 546.
Objective to investigate the protective effects of Ligustrazine preconditioning against hepatic ischemia/reperfusion injury (IRI) in rats.
Fifty male Wistar rats were randomly allocated into 3 groups: sham operation group, in which animals underwent laparotomy, experimental group and control group in which were treated with 70% IRI of the liver, especially, the animals in experimental group was given intraperitoneal injection of 2 mL Ligustrazine per day for 3 days before operation. After the operation, liver tissues were harvested at 1 h, 6 h, 24 h and 72 h for the study of histomorphological change, the respiratory control ratio (RCR) and phosphorus: oxygen ratio (P/O) of hepatocytes mitochondria, and the contents of adenosine triphosphate (ATP) of liver tissue.
(1) The damage hepatic tissue in experimental group was slighter than that in control group at each corresponding time-point after operation. (2) The RCR and P/O ratio at each corresponding time-point were higher in experimental group than those in control group (P < 0.05), and all of the two groups recovered after 72 h. (3) The ATP concentration in experimental group also was higher than that in control group at each corresponding time-point, and recovered faster than control group.
The current results show that Ligustrazine preconditioning may improve energy metabolism of rat liver in ischemia reperfusion injury.
探讨川芎嗪预处理对大鼠肝脏缺血/再灌注损伤（IRI）的保护作用。
将50只雄性Wistar大鼠随机分为3组：假手术组，即仅行剖腹术的动物组；实验组和对照组，后两组均采用70%肝脏缺血再灌注处理，特别地，实验组动物在术前3天每天腹腔注射2 mL川芎嗪。术后1 h、6 h、24 h和72 h采集肝脏组织，用于研究组织形态学变化、肝细胞线粒体的呼吸控制率（RCR）和磷氧比（P/O）以及肝脏组织三磷酸腺苷（ATP）含量。
（1）术后各相应时间点，实验组肝脏组织损伤程度均轻于对照组。（2）实验组各相应时间点的RCR和P/O比值均高于对照组（P<0.05），且两组在72 h后均恢复。（3）实验组各相应时间点的ATP浓度也高于对照组，且恢复速度比对照组快。
目前结果表明，川芎嗪预处理可能改善大鼠肝脏缺血再灌注损伤时的能量代谢。
