Zayani Yosra, Allal-Elasmi Monia, Jacob Marie Paule, Zidi Wiem, Ftouhi Bochra, Feki Moncef, Slimane Hedia, Kaabachi Naziha
L99ES11 Research Laboratory and Department of Biochemistry, Rabta University Hospital, Tunis, Tunisia.
Clin Lab. 2012;58(7-8):779-85.
The aim of this study was to determine plasma levels of matrix metalloproteinases (MMPs) 2, 3, and 9 and their tissue inhibitors (TIMPs) 1 and 2 in type 2 diabetic patients (T2DM) compared to healthy subjects.
The study involved 54 patients with T2DM and 57 age and gender matched healthy adults as controls. MMPs 2 and 9 were analyzed by gelatin zymography and MMP-3 and TIMPs 1 and 2 by ELISA.
For technical feasibility, MMPs 2 and 9 were expressed in pro forms. Pro-MMP-9 was significantly higher (p < 0.05), whereas TIMP-1 and TIMP-2 levels were significantly decreased (p < 0.01) in patients with T2DM compared to controls. The MMP-3/TIMP-1 and the MMP-3/TMP-2 ratios were significantly higher in T2DM patients than controls (p < 0.05). Fasting plasma glucose was inversely correlated with TIMP-1 (r = -0.412, p < 0.01) and TIMP-2 (r = -0.315, p < 0.001), but was not associated with MMPs.
The present study identified abnormalities in plasma markers for extracellular matrix metabolism in T2DM. The new parameters would constitute an effective approach to explore the complications of uncontrolled diabetes.
本研究旨在测定2型糖尿病患者（T2DM）与健康受试者相比血浆中基质金属蛋白酶（MMPs）2、3和9及其组织抑制剂（TIMPs）1和2的水平。
该研究纳入了54例T2DM患者和57例年龄及性别匹配的健康成年人作为对照。采用明胶酶谱法分析MMPs 2和9，采用酶联免疫吸附测定法分析MMP-3以及TIMPs 1和2。
出于技术可行性考虑，MMPs 2和9以酶原形式表达。与对照组相比，T2DM患者的前MMP-9显著升高（p < 0.05），而TIMP-1和TIMP-2水平显著降低（p < 0.01）。T2DM患者的MMP-3/TIMP-1和MMP-3/TMP-2比值显著高于对照组（p < 0.05）。空腹血糖与TIMP-1（r = -0.412，p < 0.01）和TIMP-2（r = -0.315，p < 0.001）呈负相关，但与MMPs无关。
本研究发现T2DM患者细胞外基质代谢的血浆标志物存在异常。这些新参数将构成探索未控制糖尿病并发症的有效方法。
