Ladefoged J, Langhoff E
Medical Department P, Rigshospitalet, Copenhagen, Denmark.
Kidney Int. 1990 Jan;37(1):126-30. doi: 10.1038/ki.1990.17.
Mononuclear cells (PBMC) were isolated from dialyzed patients and healthy control subjects. Lymphocyte responses to stimulation with optimal and suboptimal concentrations of lectin (PHA), or stimulation with T cell receptor antibody (Leu 4) were found decreased in the patient cultures. The separate and the combined effects of exogenous interleukin-1 (IL-1) and interleukin-2 (IL-2) were examined in PHA and Leu 4 stimulated cell cultures. Addition of IL-1 did not normalize the decreased proliferation response of the patient cultures. In contrast, addition of IL-2 alone clearly enhanced and almost normalized the response of patient cultures stimulated with suboptimal concentrations of PHA. The combined addition of IL-1 and IL-2 gave no evidence of an additive effect of IL-1 and IL-2. Cell cultures from uremic and normal HLA-identical relative were examined. Substitution of uremic adherent monocytes with normal adherent monocytes as accessory cells did not improve the uremic T cell responses to stimulation with PHA. Furthermore, uremic adherent cells did not suppress the normal T cell responses. These results suggest that uremic accessory cells support T cell activation and, in particular, do not suppress T cell responses. The effect of IL-2 in the present study as well as previous findings of decreased IL-2 production in patients cultures may indicate that uremia primarily influences the proliferation of T cells.
从透析患者和健康对照者中分离出单核细胞（PBMC）。发现在患者培养物中，淋巴细胞对最佳和次最佳浓度凝集素（PHA）刺激的反应，或对T细胞受体抗体（Leu 4）刺激的反应降低。在PHA和Leu 4刺激的细胞培养物中检测了外源性白细胞介素-1（IL-1）和白细胞介素-2（IL-2）的单独及联合作用。添加IL-1并未使患者培养物中降低的增殖反应恢复正常。相反，单独添加IL-2明显增强了用次最佳浓度PHA刺激的患者培养物的反应，并且几乎使其恢复正常。联合添加IL-1和IL-2未显示出IL-1和IL-2的相加作用。检测了尿毒症患者和正常的 HLA 相同亲属的细胞培养物。用正常贴壁单核细胞替代尿毒症贴壁单核细胞作为辅助细胞并不能改善尿毒症T细胞对PHA刺激的反应。此外，尿毒症贴壁细胞并未抑制正常T细胞反应。这些结果表明，尿毒症辅助细胞支持T细胞活化，特别是不抑制T细胞反应。本研究中IL-2的作用以及先前在患者培养物中发现的IL-2产生减少的结果可能表明，尿毒症主要影响T细胞的增殖。
