Langhoff E, Ladefoged J, Odum N
Int Arch Allergy Appl Immunol. 1986;81(1):5-11. doi: 10.1159/000234099.
The functional relationship in vitro between mitogen-induced lymphocyte transformation, lymphocyte response to interleukin-2 (IL-2) and steroid, and production of IL-2 was examined in patients with chronic renal failure on hemodialysis (HD) or on continuous ambulatory peritoneal dialysis (CAPD). The lymphocyte responses to optimal stimulation with phytohemagglutinin, concanavalin A, and pokeweed mitogen were depressed in lymphocyte cultures from HD patients, while CAPD lymphocyte cultures responded normally. However, at suboptimal phytohemagglutinin stimulation both CAPD lymphocyte and HD lymphocyte responses were subnormal. Uremic lymphocyte cultures were more sensitive to the immunosuppressive effect of methylprednisolone. Addition of IL-2 normalized the phytohemagglutinin responses of suboptimally stimulated CAPD lymphocyte cultures and clearly improved the mitogen responses of the HD lymphocyte cultures. Furthermore, the increased uremic lymphocyte sensitivity to methylprednisolone was normalized by addition of IL-2 to the cultures. The measured IL-2 production had clearly decreased in the HD cultures after 48 h as compared to that of the control cultures. A similar but not significant trend was also seen in the CAPD cultures. Thus, it is suggested that a deficient production of IL-2 may partly explain the reduced lymphocyte response of uremic lymphocytes in vitro.
对接受血液透析（HD）或持续性非卧床腹膜透析（CAPD）的慢性肾衰竭患者，研究了丝裂原诱导的淋巴细胞转化、淋巴细胞对白介素-2（IL-2）和类固醇的反应以及IL-2产生之间的体外功能关系。HD患者淋巴细胞培养物中，淋巴细胞对植物血凝素、刀豆球蛋白A和商陆丝裂原的最佳刺激反应降低，而CAPD淋巴细胞培养物反应正常。然而，在次最佳植物血凝素刺激下，CAPD淋巴细胞和HD淋巴细胞反应均低于正常水平。尿毒症淋巴细胞培养物对甲基强的松龙的免疫抑制作用更敏感。添加IL-2可使次最佳刺激的CAPD淋巴细胞培养物的植物血凝素反应正常化，并明显改善HD淋巴细胞培养物的丝裂原反应。此外，通过在培养物中添加IL-2，尿毒症淋巴细胞对甲基强的松龙增加的敏感性恢复正常。与对照培养物相比，HD培养物中48小时后测得的IL-2产生明显减少。CAPD培养物中也观察到类似但不显著的趋势。因此，提示IL-2产生不足可能部分解释了尿毒症淋巴细胞体外淋巴细胞反应降低的原因。
