Laboratory of Cellular Hematology, Center for Biologics Evaluation and Research, FDA, Bethesda, Maryland 20852-1448, USA.
Transfusion. 2013 Jun;53(6):1178-86. doi: 10.1111/j.1537-2995.2012.03896.x. Epub 2012 Sep 24.
Platelet (PLT) storage at room temperature (RT) is limited to 5 days to prevent growth of bacteria, if present, to high levels. Storage in cold temperatures would reduce bacterial proliferation, but cold-exposed PLTs are rapidly cleared from circulation by the hepatic Ashwell-Morell (AM) receptor, which recognizes PLT surface carbohydrates terminated by β-galactose. We cycled storage temperature between 4 and 37°C to preserve PLT function and reduce bacterial growth.
Temperature-cycled (TC) human PLTs were stored at 4°C for 12 hours and then incubated at 37°C for 30 minutes before returning back to cold storage. PLTs stored at RT or at 4°C (COLD) or TC for 2, 5, and 7 days were infused into SCID mice and the in vivo recovery was determined at 5, 20, and 60 minutes after transfusion.
PLTs stored for 2 days in COLD had significantly lower in vivo recoveries than RT PLTs. TC PLTs had improved recoveries over COLD and comparable to RT PLTs. After 5- and 7-day storage, TC PLTs had better recoveries than RT and COLD PLTs. PLT surface β-galactose was increased significantly for both COLD and TC PLTs compared to RT. Blocking of the AM receptor by asialofetuin increased COLD but not TC PLT recovery.
TC cold storage may be an effective method to store PLTs without loss of in vivo recovery. The increased β-galactose exposure in TC PLTs suggests that mechanisms in addition to AM receptors may mediate clearance of cold-stored PLTs.
为了防止细菌生长到高水平，如果存在细菌，血小板（PLT）在室温（RT）下的储存时间限制为 5 天。在低温下储存可以减少细菌增殖，但暴露于低温下的 PLT 会被肝脏 Ashwell-Morell（AM）受体迅速清除，该受体识别 PLT 表面由β-半乳糖终止的碳水化合物。我们在 4°C 和 37°C 之间循环储存温度，以保持 PLT 功能并减少细菌生长。
温度循环（TC）的人 PLT 在 4°C 下储存 12 小时，然后在 37°C 下孵育 30 分钟，然后再返回低温储存。在 RT 或 4°C（COLD）或 TC 下储存 2、5 和 7 天的 PLT 输注到 SCID 小鼠中，并在输血后 5、20 和 60 分钟确定体内恢复情况。
在 COLD 中储存 2 天的 PLT 体内恢复明显低于 RT PLT。TC PLT 的恢复优于 COLD，与 RT 相当。在 5 天和 7 天储存后，TC PLT 的恢复优于 RT 和 COLD PLT。与 RT 相比，COLD 和 TC PLT 的 PLT 表面β-半乳糖显著增加。用去唾液酸胎球蛋白阻断 AM 受体增加了 COLD 但不增加 TC PLT 的恢复。
TC 冷藏可能是一种有效的储存 PLT 方法，而不会损失体内恢复。TC PLT 中β-半乳糖暴露增加表明，除 AM 受体外，还有其他机制介导低温储存的 PLT 清除。
