Laboratory of Veterinary Microbiology, Veterinary Teaching Hospital, Universidade de Cuiaba, 3100 Beira Rio Ave., 78065-900 Cuiaba, MT, Brazil.
Vet Microbiol. 2013 Feb 22;162(1):207-13. doi: 10.1016/j.vetmic.2012.08.030. Epub 2012 Sep 7.
This report describes the complete genomic sequence and taxonomic position of BPV type 13. The BPV13 genome was amplified using the multiply primed rolling-circle amplification technique and long-template PCR employing two specific primers. The two long PCR fragments obtained were cloned and sequenced via primer walking. The complete genomic sequence of the BPV13 contains 7961 bp encoding eight proteins, E1, E2, E4, E5, E6, E7, L1, and L2. Similarly to the E5 gene in BPVs 1 and 2, the putative BPV13 E5 ORF encodes a small transforming protein that contains a hydrophobic transmembrane domain. Meanwhile, the retinoblastoma tumor suppressor-binding domain is absent in the putative BPV13 E7 protein. The presence of these two specific molecular features has been recognized as a distinct marker for the development of fibropapilloma in artiodactyl PV-induced lesions. The phylogenetic analysis demonstrated that BPV13 is a new member of the Deltapapillomavirus genus, to be classified as the third representative of the Delta 4 species. The characterization of the genomic sequence of this novel PV will aid in the interpretation of the pathologies described to be related to this virus and provide support for the development of diagnostic tools for epidemiological surveillance of BPV13 in its potential natural hosts.
本报告描述了 BPV 型 13 的完整基因组序列和分类地位。使用多重引物滚环扩增技术和使用两个特定引物的长模板 PCR 扩增 BPV13 基因组。通过引物行走克隆和测序获得的两个长 PCR 片段。BPV13 的完整基因组序列包含 7961 个碱基对，编码 8 种蛋白质，E1、E2、E4、E5、E6、E7、L1 和 L2。与 BPV1 和 2 的 E5 基因类似，推定的 BPV13 E5 ORF 编码一个小的转化蛋白，该蛋白含有一个疏水性跨膜结构域。同时，推定的 BPV13 E7 蛋白中不存在视网膜母细胞瘤肿瘤抑制因子结合域。这两个特定分子特征的存在已被认为是在偶蹄目动物中由 PV 诱导的纤维乳头瘤病变中发展为纤维乳头瘤的一个明显标志。系统发育分析表明，BPV13 是 Deltaapapillomavirus 属的一个新成员，被分类为 Delta 4 种的第三个代表。该新型 PV 基因组序列的特征将有助于解释与该病毒相关的病理学，并为在其潜在天然宿主中对 BPV13 进行流行病学监测的诊断工具的开发提供支持。
