Melo T C, Carvalho R F, Mazzucchelli-de-Souza J, Diniz N, Vasconcelos S, Assaf S L M R, Araldi R P, Ruiz R M, Kerkis I, Beçak W, Stocco R C
Laboratório de Genética, Instituto Butantan, São Paulo, SP, Brasil.
Departamento de Botânica, Universidade Federal de Pernambuco, Recife, PE, Brasil.
Genet Mol Res. 2014 Apr 3;13(2):2458-69. doi: 10.4238/2014.April.3.18.
Bovine papillomaviruses (BPVs) are recognized as causal agents of benign and malignant tumors in cattle. Thirteen types of BPVs have already been described and classified into 3 distinct genera. Divergences in the nucleotide sequence of the L1 gene are used to identify new viral types through the employment of PCR assays with degenerated primers. In the present study, a method for identifying BPVs based on PCR-RFLP and DNA sequencing allowed the identification of a new putative Deltapapillomavirus, designated JN/3SP (JQ280500.1). The analysis of the L1 gene showed that this strain was most closely related to the BPVs -1, -2, -13 , and OaPV1 (71-73% genetic similarity). In this study, we describe the detection of this new putative Deltapapillomavirus type and verify its phylogenetic position within the genus.
牛乳头瘤病毒（BPVs）被认为是牛良性和恶性肿瘤的致病因子。已经描述了13种BPVs，并将其分为3个不同的属。通过使用简并引物进行PCR分析，利用L1基因核苷酸序列的差异来鉴定新的病毒类型。在本研究中，一种基于PCR-RFLP和DNA测序的BPVs鉴定方法使得鉴定出一种新的假定的δ乳头瘤病毒，命名为JN/3SP（JQ280500.1）。L1基因分析表明，该毒株与BPV -1、-2、-13和OaPV1关系最为密切（遗传相似性为71-73%）。在本研究中我们描述了这种新的假定δ乳头瘤病毒类型的检测，并验证了其在该属内的系统发育位置。
