Servei de Dermatologia, Facultat de Medicina, Universitat de Barcelona, Hospital del Mar, Parc de Salut Mar, Institut Hospital del Mar d'Investigacions Mèdiques, Barcelona, Spain.
J Urol. 2012 Nov;188(5):1965-71. doi: 10.1016/j.juro.2012.07.003. Epub 2012 Sep 20.
We determined MYC gene numerical aberrations and protein expression at different stages of penile squamous cell carcinoma carcinogenesis. We correlated these findings with clinicopathological parameters and HPV infection.
We evaluated 79 cases of penile squamous cell carcinoma, including 11 in situ and 68 invasive carcinomas. The MYC cytogenetic profile was evaluated by fluorescence in situ hybridization. HPV was detected by polymerase chain reaction amplification.
MYC gains were identified in 4 of 11 in situ carcinomas (36%) and 50 of 68 invasive penile squamous cell carcinomas (73%). A significant association between MYC gains, and tumor progression and poor outcome was demonstrated (p <0.05). HPV DNA was detected in 32 of 79 penile squamous cell carcinomas (39%). High risk type 16 was the most prevalent type. MYC numerical aberrations did not correlate with HPV status. A significant association between HPV and MYC protein over expression was noted. In HPV negative cases MYC gains correlated with MYC over expression.
MYC gains progressively increased during penile squamous cell carcinoma progression from in situ samples to metastases. MYC gains were an independent factor for poor prognosis. These findings were independent of HPV infection. MYC expression was increased in samples with HPV infection, probably reflecting direct activation of MYC.
我们在阴茎鳞癌癌变的不同阶段确定 MYC 基因的数值异常和蛋白表达。我们将这些发现与临床病理参数和 HPV 感染相关联。
我们评估了 79 例阴茎鳞癌病例，包括 11 例原位癌和 68 例浸润性癌。通过荧光原位杂交评估 MYC 细胞遗传学特征。通过聚合酶链反应扩增检测 HPV。
在 11 例原位癌中有 4 例（36%）和 68 例浸润性阴茎鳞癌中有 50 例（73%）存在 MYC 增益。MYC 增益与肿瘤进展和不良预后显著相关（p<0.05）。在 79 例阴茎鳞癌中有 32 例（39%）检测到 HPV DNA。高危型 16 是最常见的类型。MYC 数值异常与 HPV 状态无关。HPV 和 MYC 蛋白过表达之间存在显著关联。在 HPV 阴性病例中，MYC 增益与 MYC 过表达相关。
在从原位样本到转移的阴茎鳞癌进展过程中，MYC 增益逐渐增加。MYC 增益是预后不良的独立因素。这些发现与 HPV 感染无关。在有 HPV 感染的样本中，MYC 表达增加，可能反映了 MYC 的直接激活。
