Department of Urology, Qilu Hospital of Shandong University, Jinan, 250012, People's Republic of China,
Med Oncol. 2013 Dec;30(4):702. doi: 10.1007/s12032-013-0702-9. Epub 2013 Sep 3.
Hepatoma-derived growth factor (HDGF) has been verified to serve as a credible prognostic marker for several types of cancers, but its role in urologic carcinomas remains undetermined. In this study, we analyzed the significance of HDGF, as well as its relative factors such as vascular endothelial growth factor-A (VEGF-A) and Ki-67, in penile squamous cell carcinoma (PSCC). Formalin-fixed paraffin-embedded PSCC samples from 54 patients receiving surgery at Qilu Hospital of Shandong University were included in the retrospective study. The expressions of HDGF, VEGF-A, and Ki-67 were detected by immunohistochemistry of a non-biotin polymerized horseradish peroxidase method. The relationships between the expressions of HDGF and VEGF-A, Ki-67 were assessed. Moreover, their correlations with clinical pathologic characteristics and disease prognosis were, respectively, evaluated. HDGF, VEGF-A, and Ki-67 were positively expressed in 28 (51.9%), 29 (53.7%), and 26 (48.1%) patients, respectively. The expressions of VEGF-A and Ki-67 were closely correlated with PSCC type (P < 0.05). A statistically significant relationship between the expressions of HDGF and VEGF-A in PSCC was observed (P = 0.03). Patients with symptom interval of more than 6 months had a significantly poorer survival rate than those with symptom interval less than 6 months (43.3 vs. 70.8%, P = 0.043). Patients with positive HDGF expression also showed a significantly poorer survival rate than those with negative HDGF expression (39.3 vs. 73.1%, P = 0.013). Logistic regression demonstrated that the expression level of HDGF was an independent predictor for the prognosis of postoperative patients. The expression of HDGF significantly correlated with VEGF-A, but not Ki-67 expression. Overexpression of HDGF, rather than VEGF-A or Ki-67, was confirmed to be an independent prognosticator of poor outcome for PSCC patients.
肝癌衍生生长因子 (HDGF) 已被证实可作为多种癌症的可靠预后标志物，但它在泌尿系统癌中的作用仍未确定。在这项研究中，我们分析了 HDGF 及其相关因素，如血管内皮生长因子-A (VEGF-A) 和 Ki-67，在阴茎鳞状细胞癌 (PSCC) 中的意义。我们回顾性研究了来自山东大学齐鲁医院接受手术的 54 例 PSCC 患者的福尔马林固定石蜡包埋 PSCC 样本。通过非生物素聚合物辣根过氧化物酶方法的免疫组织化学检测 HDGF、VEGF-A 和 Ki-67 的表达。评估了 HDGF 与 VEGF-A、Ki-67 表达之间的关系。此外，还分别评估了它们与临床病理特征和疾病预后的相关性。分别有 28（51.9%）、29（53.7%）和 26（48.1%）例患者 HDGF、VEGF-A 和 Ki-67 呈阳性表达。VEGF-A 和 Ki-67 的表达与 PSCC 类型密切相关（P < 0.05）。在 PSCC 中观察到 HDGF 和 VEGF-A 的表达之间存在统计学显著关系（P = 0.03）。症状间隔超过 6 个月的患者的生存率明显低于症状间隔小于 6 个月的患者（43.3%对 70.8%，P = 0.043）。HDGF 表达阳性的患者的生存率也明显低于 HDGF 表达阴性的患者（39.3%对 73.1%，P = 0.013）。逻辑回归表明，HDGF 的表达水平是术后患者预后的独立预测因子。HDGF 的表达与 VEGF-A 显著相关，但与 Ki-67 表达无关。HDGF 的过表达，而不是 VEGF-A 或 Ki-67，被证实是 PSCC 患者不良预后的独立预测因子。
